Effects of low dose radiation on immune cells subsets and cytokines in mice
Effects of low dose radiation on immune cells subsets and cytokines in mice
复制标题
低剂量辐射对小鼠免疫细胞亚群和细胞因子的影响
DOI：
10.1093/toxres/tfaa017
复制
发表时间：
2020-06-01
影响因子：
2.1
通讯作者：
Zhou, Ping-Kun
中科院分区：
文献类型：
--
作者：
Liu, Xiaochang;Liu, Zheng;Zhou, Ping-Kun
由于诊断成像程序、职业危害和辐射事故，全身暴露于低剂量辐射是一个令人关切的问题。在这项研究中，我们分析了单次和长期低剂量照射对免疫系统的影响。雄性Balb/c小鼠接受单次全身剂量的照射（0.01、0.05、0.2、0.5或1戈伊）。对于长期照射，在6周的时间内对小鼠照射10次（总剂量为0.2、0.5或1戈伊）。单次或长期照射后2d，脾脏巨噬细胞、自然杀伤细胞和树突状细胞数量减少，脾脏脏器系数降低。长期低剂量照射后2天，小鼠外周血中的白色细胞数量减少。长期低剂量照射后7 ~ 14天，胸腺和脾脏中免疫细胞数量开始增加，然后趋于稳定。Th 1/Th 2细胞因子和活性氧相关蛋白先下降后上升至平台期。我们的研究结果表明，单次和长期低剂量照射对免疫系统的影响有显着差异。
Whole-body exposure to low-dose radiation due to diagnostic imaging procedures, occupational hazards and radiation accidents is a source of concern. In this study, we analyzed the effects of single and long-term low-dose irradiation on the immune system. Male Balb/c mice received a single whole-body dose of irradiation (0.01, 0.05, 0.2, 0.5 or 1 Gy). For long-term irradiation, mice were irradiated 10 times (total dose of 0.2, 0.5 or 1 Gy) over a period of 6 weeks. Two days after single or long-term irradiation, the numbers of splenic macrophages, natural killer cells and dendritic cells were reduced, and the spleen organ coefficient was decreased. At 2 Days after long-term low-dose irradiation, the number of white blood cells in the peripheral blood of the mice decreased. Between 7 and 14 Days after long-term low-dose irradiation, the number of immune cells in the thymus and spleen began to increase and then stabilized. Th1/Th2 cytokines and reactive oxygen species-related proteins first decreased and then increased to a plateau. Our results show a significant difference in the effects of single and long-term low-dose irradiation on the immune system.