Matrix-Assisted Pulsed laser Evaporation-deposited Rapamycin Thin Films Maintain Antiproliferative Activity
Matrix-Assisted Pulsed laser Evaporation-deposited Rapamycin Thin Films Maintain Antiproliferative Activity
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DOI：
10.18063/ijb.v6i1.188
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发表时间：
2020-01-01
影响因子：
8.4
通讯作者：
Narayan, Roger J.
中科院分区：
文献类型：
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作者：
Cristescu, Rodica;Negut, Irina;Narayan, Roger J.
基质辅助脉冲激光蒸发（MAPLE）具有优于常规方法的许多益处（例如，浸涂、旋涂和Langmuir-Blodgett浸涂），用于在医疗器械上制造含有药理学试剂的涂层。特别地，可以严格控制施加到医疗装置表面的涂层的厚度。在这项研究中，MAPLE是用来存款雷帕霉素-聚乙烯吡咯烷酮（雷帕霉素-PVP）薄膜硅和硼硅酸盐光学玻璃基板上。Alcohol Blue和PicoGreen研究用于测量L929小鼠成纤维细胞的代谢健康和DNA含量，分别作为存活力和增殖的量度。与硼硅酸盐玻璃对照相比，MAPLE沉积的雷帕霉素-PVP表面上的细胞表现出70.6%的活力和53.7%的增殖。这些数据表明，雷帕霉素的抗增殖特性在MAPLE沉积后得以维持。
Matrix-assisted pulsed laser evaporation (MAPLE) has many benefits over conventional methods (e.g., dip-coating, spin coating, and Langmuir-Blodgett dip-coating) for manufacturing coatings containing pharmacologic agents on medical devices. In particular, the thickness of the coating that is applied to the surface of the medical device can be tightly controlled. In this study, MAPLE was used to deposit rapamycin-polyvinylpyrrolidone (rapamycin-PVP) thin films onto silicon and borosilicate optical glass substrates. Alamar Blue and PicoGreen studies were used to measure the metabolic health and DNA content of L929 mouse fibroblasts as measures of viability and proliferation, respectively. The cells on the MAPLE-deposited rapamycin-PVP surfaces exhibited 70.6% viability and 53.7% proliferation compared to a borosilicate glass control. These data indicate that the antiproliferative properties of rapamycin were maintained after MAPLE deposition.