Methadone enhances the effectiveness of 5-aminolevulinic acid-based photodynamic therapy for squamous cell carcinoma and glioblastoma in vitro
Methadone enhances the effectiveness of 5-aminolevulinic acid-based photodynamic therapy for squamous cell carcinoma and glioblastoma in vitro
复制标题
美沙酮增强基于 5-氨基乙酰丙酸的光动力疗法对鳞状细胞癌和胶质母细胞瘤的体外疗效
DOI：
10.1002/jbio.201800468
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发表时间：
2019
影响因子：
2.8
通讯作者：
Sroka Ronald
中科院分区：
文献类型：
--
作者：
Shi Lei;Buchner Alex;er;Pohla Heike;Pongratz Thomas;Ruehm Adrian;Zimmermann Wolfgang;Gederaas Odrun A;Zhang Linglin;Wang Xiuli;Stepp Herbert;Sroka Ronald
尽管已经显示出有希望的临床结果，但基于5-氨基乙酰丙酸的光动力疗法（ALA-PDT）对鳞状细胞癌（SCC）和胶质母细胞瘤的有效性仍有待提高。止痛药美沙酮能使各种肿瘤对化疗敏感。在这项体外研究中，研究了美沙酮对ALA-PDT治疗SCC（FADU）和胶质母细胞瘤（A172）的有效性的影响，包括原卟啉IX（PpIX）荧光、存活率、细胞凋亡和细胞周期阶段，每一个都有或没有美沙酮的存在。美沙酮可增加FADU细胞PpIX的产生，而减少A172细胞PpIX的产生。ALA-PDT处理的两种细胞系的存活率通过与美沙酮的组合而显著降低（P<0.05）。美沙酮还显著增加了凋亡细胞的百分比，并改善了ALA-PDT对G 0/G1期细胞周期阻滞的作用（P<0.05）。本研究证明了美沙酮影响ALA-PDT对SCC和胶质母细胞瘤细胞系的细胞毒性作用的潜力。
Although having shown promising clinical outcomes, the effectiveness of 5‐aminolevulinic acid‐based photodynamic therapy (ALA‐PDT) for squamous cell carcinoma (SCC) and glioblastoma remains to be improved. The analgesic drug methadone is able to sensitize various tumors to chemotherapy. In this in vitro study, the influence of methadone to the effectiveness of ALA‐PDT for SCC (FADU) and glioblastoma (A172) was investigated on the protoporphyrin IX (PpIX) fluorescence, survival rates, apoptosis, and cell cycle phase, each with or without the presence of methadone. The production of PpIX was increased by methadone in FADU cells while it was decreased in A172 cells. The survival rates of both cell lines treated by ALA‐PDT were significantly reduced by the combination with methadone (P< .05). Methadone also significantly increased the percentage of apoptotic cells and improved the effect of ALA‐PDT on the cell cycle phase arrest in the G0/G1 phase (P< .05). This study demonstrates the potential of methadone to influence the cytotoxic effect of ALA‐PDT for both SCC and glioblastoma cell lines.