A dog model using an implanted system for protracted hepatic arterial chemotherapy.
A dog model using an implanted system for protracted hepatic arterial chemotherapy.
复制标题
使用植入系统进行长期肝动脉化疗的狗模型。
DOI：
10.1016/0022-4804(86)90169-1
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Ensminger,WD
中科院分区：
文献类型：
--
作者：
Wollner,IS;Knutsen,CA;Ullrich,KA;Niederhuber,JE;Crudup,JW;Juni,JE;Warber,SL;Gyves,J;Stetson,PL;Ensminger,WD
一种利用大型犬和植入式输液泵进行肝动脉化疗研究的模型已经开发出来。用99M大分子聚集白蛋白进行肝动脉灌注显像，可在活体内实现肝脏的近完全灌注(~gt；90%)。该系统是可靠的，已在13只狗身上使用了1353天，平均104天，范围52-239天。根据手术前后碱性磷酸酶和血清谷丙转氨酶的测定，植入泵不会造成明显的急性肝损伤，也不会影响动物的一般活动或行为。仔细放置导管和注意所装载溶液的物理化学性质是该模型成功的因素。该模型允许在同一设备中对新的或先前存在的化疗药物进行全面的临床前药代动力学和毒理学研究，这些药物将用于以后的人体给药。通过提供研究和开发新的治疗方式的手段，它使设计更有效的针对肿瘤血管床的细胞毒疗法成为可能。
A model for hepatic arterial chemotherapy studies using large dogs and an implantable infusion pump has been developed. Using this technique near complete perfusion (>90%) of the liver can be achievedin vivoas determined by hepatic arterial perfusion scintigraphy with technitium 99m macroaggregated albumin. The system is reliable and has been in use for a total of 1353 days (mean of 104 days, range 52–239) in 13 dogs. Pump implantation causes no apparent acute liver damage based on pre- and post-operative alkaline phosphatase and serum glutamic-pyruvic transaminase determinations and does not affect the general mobility or behavior of the animals. Careful placement of the catheter and attention to the physicochemical properties of the solutions loaded are factors contributing to the success of the model. The model permits comprehensive preclinical pharmacokinetic and toxicologic studies of new or preexistent chemotherapeutic agents in the same device that will be used for later administration in human subjects. By providing the means to examine and develop new treatment modalities, it enables the design of even more potent cytotoxic therapy directed into the tumor vascular bed.