Alimentary lipemia enhances the membrane expression of platelet P-selectin without affecting other markers of platelet activation
Alimentary lipemia enhances the membrane expression of platelet P-selectin without affecting other markers of platelet activation
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DOI：
10.1016/s0021-9150(97)00260-8
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发表时间：
1998-03-01
期刊：
影响因子：
5.3
通讯作者：
Hjemdahl, P
中科院分区：
文献类型：
--
作者：
Bröijersén, A;Karpe, F;Hjemdahl, P
本研究的目的是确定是否饮食性脂血症改变体内血小板活性。血脂正常的志愿者给予脂肪餐，并在餐前、餐后3小时和6小时评估血小板功能。使用全血流式细胞术（血小板P-选择素和纤维蛋白原结合的表达）、离体过滤法（反映体内血小板聚集性）和通过测量血浆中的血小板特异性产物（β-血小板球蛋白和血小板因子4）来测定血小板聚集性和分泌。血浆甘油三酯从0.8（0.6：1.1;中位数，25和75分位数）增加至3小时时的1.7（1.0：2.3）mmol/l，并在6小时后恢复至基线水平（P < 0.001，单因素方差分析）。餐后3 h，Sf 60-400组分（大VLDL，两者P < 0.001）的载脂蛋白B-100和载脂蛋白B-48均显著升高，而Sf 20-60（小VLDL）和Sf 12-20组分（IDL）无变化。血小板功能测定显示，在未刺激的样品中，表达P-选择素的血小板百分比在餐后3 h增加40%（5%; 64%），在餐后6 h增加51%（-7%; 85%）（两者均P < 0.05）。在ADP体外刺激的样品中，在0.1 μ M下，3 h后P-选择素表达增加45%（6%; 58%），6 h后增加30%（12%; 58%）（两者P < 0.01）。血小板P-选择素表达的影响较小，在较高的ADP浓度。β-血小板球蛋白（约20 ng/ml）和血小板因子4（约0.3 ng/ml）的血浆水平不受脂肪负荷的影响。纤维蛋白原结合的流式细胞仪分析和过滤测量也未能揭示任何餐后改变。目前发现血小板P-选择素表达增强，提示血小板在餐后轻度致敏。这是否对血栓形成和动脉粥样硬化有重要意义还需要进一步研究。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
The present study was conducted to determine whether alimentary lipemia alters platelet activity in vivo. Normolipidemic volunteers were given a fatty meal and platelet function was assessed before, and 3 and 6 h after the meal. Platelet aggregability and secretion was determined using whole blood flow cytometry (expression of platelet P-selectin and fibrinogen binding), filtragometry ex vivo (reflecting platelet aggregability in vivo) and by measurements of platelet specific products in plasma (beta-thromboglobulin and platelet factor 4). Plasma triglycerides increased from 0.8 (0.6:1.1; median, 25th and 75th percentiles) to 1.7 (1.0:2.3) mmol/l at 3 h and returned to baseline after 6 h (P < 0.001, one-way ANOVA). Apo B-100 and apo B-48 were both markedly increased 3 h postprandially in the Sf 60-400 fraction (large VLDLs, P < 0.001 for both), whereas the Sf 20-60 (small VLDLs) and Sf 12-20 fractions (IDL) did not change. The platelet function assessments revealed that the percentage of platelets expressing P-selectin increased by 40% (5%; 64%) after 3 h and by 51% (-7%; 85%) 6 h postprandially in unstimulated samples (P < 0.05 for both). In samples stimulated by ADP in vitro P-selectin expression increased by 45% (6%; 58%) after 3 h and by 30% (12%; 58%) (P < 0.01 for both) after 6 h at 0.1 mu M. Platelet P-selectin expression was less influenced at higher ADP concentrations. The plasma levels of beta-thromboglobulin (approximate to 20 ng/ml) and platelet factor 4 (approximate to 0.3 ng/ml) were not affected by the fat load. Flow cytometric analyses of fibrinogen binding and filtragometry measurements also failed to reveal any postprandial alterations. The present finding of enhanced platelet P-selectin expression suggests that platelets are mildly sensitized postprandially. Whether this is of importance for thrombus formation and atherosclerosis needs to be studied further. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.