Feasibility of pedicled vascularized inguinal lymph node transfer in a mouse model: A preliminary study
Feasibility of pedicled vascularized inguinal lymph node transfer in a mouse model: A preliminary study
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DOI：
10.1002/micr.30394
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发表时间：
2019-03-01
期刊：
影响因子：
2.1
通讯作者：
Yamamoto, Yuhei
中科院分区：
文献类型：
--
作者：
Ishikawa, Kosuke;Maeda, Taku;Yamamoto, Yuhei
目的血管化淋巴结转移在淋巴水肿的治疗中越来越普遍，但缺乏合适的小动物模型。在这里，我们评估了带蒂带血管的小鼠腹股沟淋巴结转移的可行性。方法选用小鼠25只。在切除腘窝淋巴结后，将腹股沟淋巴结带血管蒂的皮瓣转移到同侧腘窝。采用吲哚菁绿（ICG）血管造影检查皮瓣血管状况。术后3、4周行ICG淋巴造影评估淋巴流量。术后4周，在同侧后爪注射深蓝色染料，观察转移淋巴结的染色情况。然后收集所有转移的淋巴结，并用苏木精和伊红染色进行组织学评估。结果在25只小鼠中，有16只小鼠的ICG淋巴图显示转移的淋巴结与传入淋巴管之间有重新连接，蓝色染色证实了这一点。组织学上，有传入淋巴连接的转移淋巴结大小明显缩小（0.370.24mm(2)），并有明显的滤泡形成，而没有传入淋巴连接的转移淋巴结大小缩小较小（1.31 +/- 1.17mm(2)）；细胞群过于密集，无法识别卵泡。结论建立了小鼠血管化淋巴结转移模型，传入淋巴再连接可预测。血管化和再连接对于转移淋巴结的功能再生都是必要的。
PurposeVascularized lymph node transfer is becoming more common in the treatment of lymphedema, but suitable small animal models for research are lacking. Here, we evaluated the feasibility of pedicled vascularized inguinal lymph node transfer in mice.MethodsTwenty-five mice were used in the study. An inguinal lymph node-bearing flap with a vascular pedicle containing the superficial caudal epigastric vessels was transferred into the ipsilateral popliteal fossa after excision of the popliteal lymph node. Indocyanine green (ICG) angiography was used to confirm vascularity of the flap. ICG lymphography was performed to evaluate lymphatic flow at 3 and 4 weeks postoperatively. Patent blue dye was injected into the ipsilateral hind paw to observe staining of the transferred lymph node at 4 weeks postoperatively. All transferred lymph nodes were then harvested and histologically evaluated by hematoxylin and eosin staining.ResultsIn 16 of the 25 mice, ICG lymphography showed reconnection between the transferred lymph node and the afferent lymphatic vessels, as confirmed by patent blue staining. Histologically, these transferred lymph nodes with afferent lymphatic reconnection significantly regressed in size (0.370.24mm(2)) and showed clear follicle formation, whereas those without afferent lymphatic reconnection showed less size regression (1.31 +/- 1.17mm(2)); the cell population was too dense to allow identification of follicles.ConclusionsWe established a mouse model of vascularized lymph node transfer with predictable afferent lymphatic reconnection. Both the vascularization and reconnection might be necessary for functional regeneration of the transferred lymph node.