Increased expression of Fractalkine is correlated with a better prognosis and an increased number of both CD8+ T cells and natural killer cells in gastric adenocarcinoma
Increased expression of Fractalkine is correlated with a better prognosis and an increased number of both CD8+ T cells and natural killer cells in gastric adenocarcinoma
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DOI：
10.1245/s10434-008-9876-3
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发表时间：
2008-06-01
影响因子：
3.7
通讯作者：
Tanaka, Tsuneo
中科院分区：
文献类型：
--
作者：
Hyakudomi, Miki;Matsubara, Takeshi;Tanaka, Tsuneo
背景：Fractalkine （CX3CL1）是唯一一种能化学吸引自然杀伤细胞（NK）、CD8(+) T细胞、单核细胞和树突状细胞的CX3C趋化因子。虽然实验研究表明Fractalkine在肿瘤细胞中的表达与浸润淋巴细胞有关，并启动抗肿瘤免疫，但Fractalkine在胃腺癌中的临床意义尚待阐明。方法：对158例根治性切除的T2或T3型胃腺癌组织切片进行Fractalkine免疫组化染色。此外，为了评估CD8(+) T细胞和NK细胞的浸润情况，分别使用CD8和CD57蛋白抗体进行免疫组化。结果：经Spearman秩相关系数检验，Fractalkine评分与CD8(+) T细胞和NK细胞数量呈正相关（P = 0.0080, P < 0.05）。0031年,分别)。此外，高Fractalkine表达组（n = 67）在无病生存方面的预后明显优于低Fractalkine表达组（n = 91） （P = 0.0016）。在多变量分析中，Fractalkine表达被确定为无病生存的独立预后因素之一（风险比，2.5;P = 0.0147）。结论：这些数据提示肿瘤细胞表达Fractalkine可增强CD8(+) T细胞和NK细胞的募集，诱导先天免疫和适应性免疫，从而改善胃腺癌的预后。Fractalkine是一种新的独立预后预测因子，可以作为开发更有效的胃腺癌治疗策略的新候选物。
Background: Fractalkine (CX3CL1) is the only CX3C chemokine that can chemoattract natural killer (NK) cells, CD8(+) T cells, monocytes, and dendritic cells. Although experimental studies have demonstrated that Fractalkine expression by tumor cells is related to the infiltrating lymphocytes and initiates antitumor immunity, the clinical significance of Fractalkine remains to be elucidated in gastric adenocarcinoma.Methods: Tissue sections from 158 patients with curatively resected T2 or T3 gastric adenocarcinoma were immunohistochemically stained for Fractalkine. Furthermore, to evaluate CD8(+) T cells and NK cells infiltration, antibodies to CD8 and CD57 protein were respectively used for immunohistochemistry.Results: A significant direct correlation was observed between the Fractalkine scores and the number of CD8(+) T cells and NK cells using the Spearman rank correlation coefficient test (P = .0080, .0031, respectively). Furthermore, the high Fractalkine expression group (n = 67) showed a significantly better prognosis than the low Fractalkine expression group (n = 91) regarding the disease-free survival (P = .0016). In a multivariate analysis, the Fractalkine expression was identified as one of the independent prognosticators for disease-free survival (risk ratio, 2.5; P = .0147).Conclusions: These data suggest that the expression of Fractalkine by tumor cells enhances the recruitment of CD8(+) T cells and NK cells and induces both innate and adaptive immunity, thereby yielding a better prognosis in gastric adenocarcinoma. Fractalkine is a new independent predictor of the prognosis and can be a novel candidate for development of a more effective therapeutic strategy for gastric adenocarcinomas.