CHAPTER 15: Molecular Methods for Detection of Plant Pathogens in Irrigation Water
CHAPTER 15: Molecular Methods for Detection of Plant Pathogens in Irrigation Water
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第 15 章：检测灌溉水中植物病原体的分子方法
DOI：
10.1094/9780890544914.018
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
von Bargen
中科院分区：
文献类型：
--
作者：
von Bargen
分子技术提供了灵敏、快速和定量的分析工具，适合检测和估计病原体的数量。此外，这些技术可以区分病理变异并检测新出现的变异。 PCR 在植物病原体诊断中的成功反映在已发表的许多修改中，这些修改补充了分子检测工具的库，甚至可以用于检测水生环境中的植物病原体。此外，分子技术的改进，如多重 PCR、DNA 阵列技术和宏基因组方法中的全基因组测序，不仅能够识别大量不同的植物病原体（真菌、细菌和病毒），而且能够在单次测定中在亚种水平上分别区分菌株、病原体和种族。
Molecular techniques provide sensitive, rapid, and quantitative analytical tools that are suitable for detecting and estimating numbers of pathogens. Moreover, these techniques allow differentiation between pathovars and detection of emerging and new variants. The success of PCR in the diagnosis of plant pathogens has been mirrored by the many modifications that have been published supplementing the repertoire of molecular detection tools—even for detecting plant pathogens in aquatic environments. Furthermore, modifications of molecular techniques such as multiplex PCR, DNA array technology, and sequencing of whole genomes in a metagenomic approach provide the capability not only to identify large numbers of different plant pathogens—fungi, bacteria, and viruses—but also to differentiate strains, pathovars, and races, respectively, at the subspecies level in a single assay.