Stochastic variation in Cardamine hirsuta petal number.
Stochastic variation in Cardamine hirsuta petal number.
复制标题
碎米荠花瓣数的随机变化。
作者：
M. Monniaux;B. Pieper;Angela Hay
背景与目的
花的发育在每轮花器官的特性和数量方面是非常健壮的，而营养体的发育可以是相当可塑的。即使在波动的环境中，这种花发育的渠道化也防止了隐性遗传变异的表型表达。十字形花被有四个花瓣是拟南芥科的标志，典型的模式种是拟南芥。然而，在碎米荠中发现了可变的花瓣损失，碎米荠是A.结果表明，在随机、遗传和环境扰动的共同作用下，红花碎米荠花瓣数发生了显著的变化，这使其成为研究植物去壳机制和隐性变异表达的一个有趣模型。
方法
利用重组自交系（RILs）的多性状数量性状基因座（QTL）分析，研究了重组自交系中是否存在随机变异。多毛花瓣数有遗传基础。
关键成果
随机变异（平均花瓣数的标准误差）被认为是一个可遗传的表型，并确定了四个QTL，影响这一性状。通过考虑老化对花瓣数变化的影响，检测这些QTL效应的灵敏度增加。所有QTL均对平均花瓣数及其标准差有显著影响，表明这两个性状具有共同的遗传基础。然而，对于一些QTL，在一定程度上的独立性之间的花的年龄，等位基因的影响是显着的每一个性状。
结论
C.随机变异多毛类植物花瓣数具有遗传基础，普通QTL既影响平均花瓣数，又影响平均花瓣数的标准差。因此，这些QTL的等位基因变异可以通过对表型平均值和随机变异的影响以年龄特异性方式修改花瓣数。这些结果进行了讨论的背景下，性状的进化通过鲁棒性的损失。
BACKGROUND AND AIMS
Floral development is remarkably robust in terms of the identity and number of floral organs in each whorl, whereas vegetative development can be quite plastic. This canalization of flower development prevents the phenotypic expression of cryptic genetic variation, even in fluctuating environments. A cruciform perianth with four petals is a hallmark of the Brassicaceae family, typified in the model species Arabidopsis thaliana However, variable petal loss is found in Cardamine hirsuta, a genetically tractable relative of A. thaliana Cardamine hirsuta petal number varies in response to stochastic, genetic and environmental perturbations, which makes it an interesting model to study mechanisms of decanalization and the expression of cryptic variation.
METHODS
Multitrait quantitative trait locus (QTL) analysis in recombinant inbred lines (RILs) was used to identify whether the stochastic variation found in C. hirsuta petal number had a genetic basis.
KEY RESULTS
Stochastic variation (standard error of the average petal number) was found to be a heritable phenotype, and four QTL that influenced this trait were identified. The sensitivity to detect these QTL effects was increased by accounting for the effect of ageing on petal number variation. All QTL had significant effects on both average petal number and its standard error, indicating that these two traits share a common genetic basis. However, for some QTL, a degree of independence was found between the age of the flowers where allelic effects were significant for each trait.
CONCLUSIONS
Stochastic variation in C. hirsuta petal number has a genetic basis, and common QTL influence both average petal number and its standard error. Allelic variation at these QTL can, therefore, modify petal number in an age-specific manner via effects on the phenotypic mean and stochastic variation. These results are discussed in the context of trait evolution via a loss of robustness.
DOI：
10.1111/nph.13586
发表时间：
2016
期刊：
The New phytologist
影响因子：
--
作者：
Pieper B
通讯作者：
Pieper B