Impact of Perkinsus olseni infection on a wild population of Manila clam Ruditapes philippinarum in Ariake Bay, Japan.
Impact of Perkinsus olseni infection on a wild population of Manila clam Ruditapes philippinarum in Ariake Bay, Japan.
复制标题
奥尔森氏柏金虫感染对日本有明湾菲律宾蛤仔野生种群的影响。
DOI：
10.1016/j.jip.2018.03.001
复制
发表时间：
2018
影响因子：
3.4
通讯作者：
T. Yoshinaga
中科院分区：
文献类型：
--
作者：
T. Waki;M. Takahashi;Tatsuya Eki;Masato Hiasa;Kousuke Umeda;Nanae Karakawa;T. Yoshinaga
许多研究已经解决了菲律宾蛤仔（Ruditapes philippinarum）在日本的产量下降问题，但很少有人关注蛤仔感染Perkinsus olseni的问题。评价P. olseni，从2009年6月到2013年1月，每月或每两个月在潮滩监测一次野生未开发蛤蜊种群的感染水平和宿主密度。荧光定量PCR分析鉴别P. olseniandPerkinsushonshuensis仅检测到P.奥尔谢宁测试了蛤蜊。P.在整个研究期间，除新招募的蛤蜊外，7个队列中的olseni为100%或接近100%。直到9月份，新招募的文蛤的感染强度仍然很低，很少达到106个/g湿组织。秋季感染强度达到106个细胞/克，并保持在104- 106个细胞/克的高水平，直到每个队列的蛤蜊消失。在秋季，当感染强度达到约1000时，文蛤密度开始下降。106个/g。密度在冬季相对稳定，在春季增加，并再次下降，在夏季和秋季在1岁或以上的蛤蜊在随后的几年。实验感染P.奥尔谢尼亚特角106个/g的感染率，并在潮滩网箱中放置1 ~ 2个月，其存活率显著低于未感染对照组。结果提示，重度感染P.尽管其他环境和生物因素也可能导致密度下降，但Olseni是导致蛤密度下降的主要原因。此外，从2010年6月至2013年1月，将未感染的蛤蜊置于笼子中1-2个月，监测患病率和感染强度。夏秋季寄生虫传播和感染进展增加。
Many studies have addressed the production decline of Manila clam,Ruditapes philippinarum, in Japan, but infection of clams withPerkinsus olsenihas received scarce attention. To evaluate the impact ofP. olseni, infection levels and host density of a wild, unexploited clam population were monitored monthly or bimonthly on a tidal flat from June 2009 to January 2013. Real-time PCR analysis discriminatingP. olseniandPerkinsus honshuensisdetected onlyP. olseniin tested clams. The prevalence ofP. olseniwas 100% or nearly 100% in 7 cohorts throughout the study period, except in newly recruited clams. Infection intensity remained low and seldom reached 106cells/g wet tissue in newly recruited clams until the month of September. Infection intensity reached 106cells/g in autumn and remained high at 104–106cells/g until each cohort of clams disappeared. Clam density began to decrease in the autumn when the infection intensities reached ca. 106cells/g. Density was relatively stable in winter, increased in spring and decreased again in clams aged 1 year or older during summer and autumn in the following years. Survival of clams experimentally infected withP. olseniat ca. 106cells/g and placed in a cage in the tidal flat for 1 or 2 months was significantly lower than survival of uninfected control clams. Our results suggested that heavy infection withP. olseniwas a major cause of the clam density decrease, although other environmental and biological factors also may have contributed to the decline in density. In addition, uninfected clams were deployed in cages for 1–2 months from June 2010 to January 2013 and prevalence and infection intensity were monitored. Parasite transmission and infection progression increased in summer and autumn.
低盐度和低温对马尼拉蛤体内原生动物奥尔森氏珀金虫（Perkinsus olseni）繁殖的抑制作用
DOI：
--
发表时间：
2015
期刊：
Fish Parasitology
影响因子：
--
作者：
Tsukasa Waki;Tomoyoshi Yoshinaga
通讯作者：
Tomoyoshi Yoshinaga
幼年和成年马尼拉蛤与原生动物 Perkinsus olseni 在不同温度下的实验挑战
影响因子：
1.9
作者：
Tsukasa Waki;Tomoyoshi Yoshinaga
通讯作者：
Tomoyoshi Yoshinaga