Hsp70 expression in Enteromorpha intestinalis (Chlorophyta) exposed to environmental stressors
Hsp70 expression in Enteromorpha intestinalis (Chlorophyta) exposed to environmental stressors
复制标题
DOI：
10.1016/s0166-445x(00)00119-3
复制
发表时间：
2001-01-01
影响因子：
4.5
通讯作者：
Depledge, MH
中科院分区：
文献类型：
--
作者：
Lewis, S;Donkin, ME;Depledge, MH
大量研究表明，应激蛋白有可能作为暴露于环境污染物中的生物标志物。动物应激蛋白的分析，特别是热休克蛋白70，主导了这一领域的研究，尽管植物作为污染监测器的兴趣日益浓厚。在本研究中，我们研究了在不同的应激源下，HSP70在肠形蛔虫中的表达，以评估HSP70作为污染暴露在这种海藻中的生物标志物的潜在用途。肠草暴露于热胁迫、不同营养条件下的铜和三嗪除草剂Irgarol 1051。还进行了测量，以比较热休克蛋白70与常规亚致死毒性终点，如生长和荧光诱导参数，如Fv/Fm。肠衣原体表现出典型的热休克反应。热休克蛋白70的表达随着铜暴露的增加而增加，但与生长测量相比，证明是铜暴露的相对不敏感的生物标志物。营养限制增加了铜的毒性，并显著损害了生长、Fv/Fm和Hsp70的产生。Irgarol 1051暴露对Fv/Fm和生长有强烈影响，但Hsp70水平在暴露于除草剂后没有改变。讨论了将Hsp70在肠杆菌中的表达作为生物标志物的意义。(C)2001 Elsevier Science B.V.保留所有权利。
Numerous studies have indicated that stress proteins have potential as biomarkers of exposure to environmental contaminants. Analysis of stress proteins in animals, especially Hsp70, has dominated this research area despite increasing interest in the use of plants as pollution monitors. In the present study Hsp70 expression in Enteromorpha intestinalis has been investigated under exposure to a variety of stressors, to evaluate the potential use of Hsp70 as a biomarker of contaminant exposure in this seaweed. E. intestinalis was exposed to thermal stress, copper under differing conditions of nutrient availability and the triazine herbicide Irgarol 1051. Measurements were also taken to compare Hsp70 with conventional sublethal endpoints of toxicity such as growth and fluorescence induction parameters such as Fv/Fm. E. intestinalis exhibited a typical heat shock response. Hsp70 expression was increased with copper exposure, but proved to be a relatively insensitive biomarker of copper exposure compared to growth measurements. Nutrient limitation enhanced copper toxicity and significantly impaired growth, Fv/Fm and Hsp70 production. Fv/Fm and growth were strongly affected by Irgarol 1051 exposure, but Hsp70 levels were unaltered following exposure to the herbicide. The implications of using Hsp70 expression in E. intestinalis as a biomarker are discussed. (C) 2001 Elsevier Science B.V. All rights reserved.