Functional differences between memory and naive CD8 T cells
Functional differences between memory and naive CD8 T cells
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DOI：
10.1073/pnas.96.6.2976
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发表时间：
1999-03-16
影响因子：
11.1
通讯作者：
Chen, JZ
中科院分区：
文献类型：
--
作者：
Cho, BK;Wang, CY;Chen, JZ
为了确定小鼠记忆T细胞和幼稚T细胞有何不同，我们产生了大量的长寿命记忆CD8(+) T细胞，并将它们与表达相同抗原特异性受体（T细胞受体）的幼稚细胞进行比较。尽管这两个群体表达了相似的TCR和CDS水平，但在体外抗原刺激下，记忆T细胞比幼稚T细胞更快更广泛地下调TCR，分泌ifn - γ和IL-2的速度更快，记忆细胞也更大，当刚从小鼠中分离出来时，它们含有穿孔素，无需重新刺激就能杀死靶细胞。它们进一步不同于幼稚细胞，需要IL-15来增殖，并且在体外有更大的凋亡倾向。然而，在体内抗原刺激下，它们的增殖速度比原始细胞快。这些发现表明，与初始T细胞不同，CD8记忆T细胞具有内在的编程能力，可以在体内快速表达其效应功能，而无需经历克隆扩增和分化。
To determine how murine memory and naive T cells differ, we generated large numbers of long-lived memory CD8(+) T cells and compared them to naive cells expressing the same antigen-specific receptor (T cell receptor: TCR), Although both populations expressed similar levels of TCR and CDS, on antigen stimulation in vitro memory T cells down-regulated their TCR faster and more extensively and secreted IFN-gamma and IL-2 faster than naive T cells, Memory cells were also larger, and when freshly isolated from mice they contained perforin and killed target cells without having to be restimulated. They further differed from naive cells in requiring IL-15 for proliferation and in having a greater tendency to undergo apoptosis in vitro. On antigen stimulation in vivo, however, their proliferated more rapidly than naive cells. These findings suggest that, unlike naive T cells, CD8 memory T cells are intrinsically programmed to rapidly express their effector functions in vivo without having to undergo clonal expansion and differentiation.