AN INTEGRATED EXPERIMENTAL-STUDY OF ENDODERM FORMATION IN AVIAN EMBRYOS
AN INTEGRATED EXPERIMENTAL-STUDY OF ENDODERM FORMATION IN AVIAN EMBRYOS
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DOI：
10.1007/bf00315703
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
IRELAND, GW
中科院分区：
文献类型：
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作者：
STERN, CD;IRELAND, GW
本研究结合几种技术对鸟类胚胎原条期内胚层的形成进行了研究。这些技术包括显微外科手术、延时摄影、使用鸡-鹌鹑嵌合体、氚化胸苷放射自显影和一种新技术，用于在已知区域的小块组织在培养物中保持24小时后鉴定细胞形态。这些技术证实，早期鸡胚的腹层接受了透明区边缘和中心区域的贡献。前者似乎由来自胚壁的卵黄细胞组成;后者由来自胚胎更背层的更小、更少的卵黄细胞组成。在这些阶段中，下层向前的运动似乎取决于由更多尾部区域中的扩张组织施加于其上的机械约束。2个贡献中的每一个对下层的程度被确定为阶段和下层的存在或不存在的函数。
The formation of the endoderm during primitive streak stages in avian embryos was studied by combining several techniques for each embryo. These included microsurgery, time-lapse filming, use of chick-quail chimeras, tritiated thymidine autoradiography and a novel technique for identifying the morphology of the cells after small pieces of tissue from known areas had been maintained in culture for 24 h. These techniques confirmed that the ventral layer of the early chick embryo receives contributions from both the marginal and the central regions of the area pellucida. The former seems to consist of yolky cells derived from the germ wall; the latter consists of smaller, less yolky cells derived from the more dorsal layers of the embryo. The movement of the lower layer anteriorly during these stages appears to be dependent upon mechanical constraints imposed upon it by the expanding tissue in more caudal regions. The extent of each of the 2 contributions to the lower layer was determined as a function of stage and presence or absence of a lower layer.