Enhanced proliferation of progenitor cells following long-term potentiation induction in the rat dentate gyrus
Enhanced proliferation of progenitor cells following long-term potentiation induction in the rat dentate gyrus
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DOI：
10.1016/j.nlm.2006.05.005
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发表时间：
2006-11-01
影响因子：
2.7
通讯作者：
Jung, Min Whan
中科院分区：
文献类型：
--
作者：
Chun, Sung Kun;Sun, Woong;Jung, Min Whan
齿状回（DG）是哺乳动物大脑中为数不多的在成年后继续产生新神经元的区域之一。尽管其功能意义尚不完全清楚，但一些证据表明DG神经发生在学习和记忆中的作用。考虑到长期增强（LTP）是海马学习和记忆过程的主要候选过程，这些结果提出了LTP和神经发生密切相关的可能性。在这里，我们研究了LTP在传入通路中的诱导是否会引发DG中祖细胞的增殖增强。通过强电刺激一侧半球穿孔通路-DG突触诱导LTP，并定量DG中新生成祖细胞（brdu标记）的数量。与对照脑半球（低频脉冲刺激）相比，ltp诱导的脑半球在DG背侧和腹侧的新生成祖细胞数量显著增加。当给药CPP（一种NMDA受体拮抗剂）时，破伤风刺激既没有诱导LTP，也没有增强祖细胞的增殖，这表明NMDA受体的激活，而不是破伤风刺激本身，是促进对照动物祖细胞增殖的原因。我们的研究结果表明，足以诱导UP的穿孔通路的破伤风刺激以NMDA受体依赖的方式增加成人DG的祖细胞增殖。(c) 2006爱思唯尔公司版权所有。
The dentate gyrus (DG) is among the few areas in the mammalian brain where production of new neurons continues in the adulthood. Although its functional significance is not completely understood, several lines of evidence suggest the role of DG neurogenesis in learning and memory. Considering that long-term potentiation (LTP) is a prime candidate for the process underlying hippocampal learning and memory, these results raise the possibility that LTP and neurogenesis are closely related. Here, we investigated whether or not LTP induction in the afferent pathway triggers enhanced proliferation of progenitor cells in the DG. LTP was induced by tetanic stimulation in perforant path-DG synapses in one hemisphere, and the number of newly generated progenitor (BrdU-labeled) cells in the DG was quantified. Compared with the control hemisphere (stimulated with low-frequency pulses), the LTP-induced hemisphere contained a significantly higher number of newly generated progenitor cells in the dorsal as well as ventral DG. When CPP, an NMDA receptor antagonist, was administered, tetanic stimulation neither induced LTP nor enhanced progenitor cell proliferation, indicating that NMDA receptor activation, rather than tetanic stimulation per se, is responsible for enhanced progenitor proliferation in the control animal. Our results show that tetanic stimulation of perforant path sufficient to induce UP increases progenitor proliferation in adult DG in an NMDA receptor-dependent manner. (c) 2006 Elsevier Inc. All rights reserved.