Nationwide Surveillance of 18 Respiratory Viruses in Patients With Influenza-Like Illnesses: A Pilot Feasibility Study in the French Sentinel Network
Nationwide Surveillance of 18 Respiratory Viruses in Patients With Influenza-Like Illnesses: A Pilot Feasibility Study in the French Sentinel Network
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DOI：
10.1002/jmv.22113
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发表时间：
2011-08-01
影响因子：
12.7
通讯作者：
Carrat, Fabrice
中科院分区：
文献类型：
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作者：
Falchi, Alessandra;Turbelin, Clement;Carrat, Fabrice
本研究的目的是测试利用PCR-DNA微阵列检测方法将18种呼吸道病毒的诊断整合到流感样疾病的临床监测中的可行性。这项研究是在法国哨兵网络中进行的，这是一个全国性的全科医生监测网络，代表了法国全科医生的年龄、地点和执业类型（城市/农村）。三个病毒学实验室也参与了这项研究。研究计划从2010年1月25日至2010年2月27日，为期5周。在法国大陆招募了150名哨兵全科医生，收集了每周因流感样疾病就诊的第一位患者的临床数据和鼻咽样本，流感样疾病的定义是39摄氏度或更高的突然发烧，伴有呼吸道症状和肌痛。63名全科医生（42%）采集103份样本，87名全科医生（58%）未采集。全科医生在年龄、性别、城市/农村分布或在哨兵网络中登记的年限方面没有差异。纳入的患者年龄相仿，具有相似的疫苗接种特征，但在研究期间向网络报告的流感样疾病患者中，男性更常见。96份可解释样本中的56份（58%）检测到61种病毒。呼吸道病毒以偏肺病毒和呼吸道合胞病毒检出最多。本研究表明，18种呼吸道病毒的病毒学诊断可与临床流感样疾病的监测相结合。尽管可行性尚未得到证实，但将在2010-2011年冬季对其进行评估。中华医学杂志，2011,33(3):551 - 557。(C) 2011 Wiley-Liss, Inc。
The aim of the present study was to test the feasibility of integrating the diagnosis of 18 respiratory viruses into clinical surveillance of influenza-like illness using a PCR-DNA microarray detection assay. The study took place in the French Sentinel Network, a nationwide surveillance network of General Practitioners (GPs) representative of French GPs in terms age, location, and type of practice (urban/rural). Three virological laboratories also participated in the study. The study was planned for 5 weeks from January 25, 2010 to February 27, 2010. A subset of 150 Sentinel GPs, located in mainland France, was enrolled to collect clinical data and nasopharyngeal samples from every first patient of the week having a medical visit for influenza-like illness defined as a sudden fever of 39 C or more with respiratory symptoms and myalgia. Sixty-three GPs (42%) collected 103 samples while 87 GPs (58%) did not. GPs did not differ with respect to their age, gender, urban/rural distribution, or years of inscription in the Sentinel Network. Patients included were of a similar age and had similar vaccination characteristics, but were more frequently men than influenza-like illness patients reported to the network during the study period. Sixty-one viruses were detected from 56 of 96 (58%) interpretable samples. The respiratory viruses detected most frequently were metapneumovirus and respiratory syncytial virus. This study showed that virological diagnosis of 18 respiratory viruses can be combined with surveillance of clinical influenza-like illness in general practice. Although feasibility has not been demonstrated yet, it will be evaluated over the winter of 2010-2011. J. Med. Virol. 83:1451-1457, 2011. (C) 2011 Wiley-Liss, Inc.