Manipulation of cell-sized phospholipid-coated microdroplets and their use as biochemical microreactors
Manipulation of cell-sized phospholipid-coated microdroplets and their use as biochemical microreactors
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DOI：
10.1021/la0618521
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发表时间：
2007-01-16
期刊：
影响因子：
3.9
通讯作者：
Yoshikawa, K.
中科院分区：
文献类型：
--
作者：
Hase, M.;Yamada, A.;Yoshikawa, K.
细胞大小的水滴被覆盖着一层模仿活细胞内表面的磷脂层，由激光镊子操纵，并用作生化微反应器。细胞大小的磷脂包裹微滴(CPMD)由直径1-100µm的矿物油中的水滴组成，并被1，2-dioleoyl-sn-glycero-3-phosphoethanolamine.包覆我们监测了分别含有底物和酶的两个CPMD控制融合后得到的单个CPMD中生化反应的时间发展。我们介绍了两个酶反应的结果：在酯酶存在下产生钙黄绿素和绿色荧光蛋白的表达。
Cell-sized water droplets coated by a phospholipid layer mimicking the inner surface of living cells were manipulated by laser tweezers and used as biochemical microreactors. The cell-sized phospholipid-coated microdroplets (CPMDs) consisted of a water droplet in mineral oil with a diameter of 1-100 mu m and coated by 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. We monitored the time development of biochemical reactions in a single CPMD obtained after the controlled fusion of two CPMDs containing a substrate and an enzyme, respectively. We present results on two enzymatic reactions: calcein production in the presence of esterase and green fluorescence protein expression.