Spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates.
Spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates.
复制标题
自发突变导致水疱性口炎病毒野外分离株糖蛋白的抗原变异。
DOI：
10.1016/0042-6822(90)90055-v
复制
发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Wagner,RR
中科院分区：
文献类型：
--
作者：
Luo,LZ;Li,Y;Snyder,RM;Wagner,RR
Strains of vesicular stomatitis virus, New Jersey serotype (VSV-NJ), isolated from diseased cattle or swine were examined by genomic RNA sequencing for genetic diversity potentially leading to antigenic variations in their type-specific glycoproteins as determined by reactivity with epitope-specific monoclonal antibodies (MAbs). Seven field isolates recovered in Colorado, New Mexico, Georgia, and Mexico during the widespread 1982–1985 epizootic in the western United States resembled the prototypic 1952 Hazelhurst subtype by partial sequence homology, but amino acid reversions to the 1949 Ogden subtype occurred frequently. When studies were performed with MAbs directed to the Ogden subtype glycoprotein, relatively limited antigenic variation, and only in neutralization epitope VIII, was noted among two of five epizootic isolates from Colorado and New Mexico. However, amino acid differences in the glycoprotein of a 1983 isolate from an enzootic region of Georgia resulted in major antigenic deficiencies in epitopes V, VI, and VII as determined by Western blotting and neutralization of infectivity with epitope-specific MAbs. Quite a few genetic but no antigenic differences were noted in an enzootic 1984 isolate from Mexico, a potential origin of the United States epizootic. Marked or complete loss of epitopes VII, VI, VIII, and V can be traced to spontaneous mutations leading to amino acid substitutions at glycoprotein positions 199, 263, 275, and 317, respectively, in the enzootic Georgia isolate 07/83-GA-P and the epizootic New Mexico isolate 06/85-NM-B. By comparison, closely adjacent amino acid substitutions at glycoprotein positions 210, 268, 277, and 364 occurred in epitope-deficient mutants selected for resistance to neutralization by MAbs specific for epitopes VII, VI, VIII, and V, respectively. Two neutralization epitopes designated X and XI were found to be unique for the G protein of the 1952 Hazelhurst isolate … /52-GA-P. The epitope X-specific MAb H21, in particular, failed to neutralize the infectivity not only of the Ogden subtype …/49-UT-B but also was ineffective against all the 1982–1985 field isolates. The classical 1952 Hazelhurst strain of VSV-NJ is genetically and antigenically quite different from those viruses isolated during the 1982–1985 epizootic.
登录
查看更多内容
水疱性口炎病毒（新泽西血清型）糖蛋白基因的点突变通过表位特异性单克隆抗体的中和抗性进行选择。
影响因子：
3.7
作者：
Luo,LH;Li,Y;Snyder,RM;Wagner,RR
通讯作者：
Wagner,RR
影响因子：
3.7
作者：
NICHOL, ST;ROWE, JE;FITCH, WM
通讯作者：
FITCH, WM
新泽西州水泡性口炎病毒的分子流行病学和进化
影响因子：
5.4
作者：
S. Nichol
通讯作者：
S. Nichol
水泡性口炎病毒（新泽西血清型）基质蛋白 mRNA 的完整核苷酸序列。
影响因子：
3.7
作者：
Dalip S. Gill;A. Banerjee
通讯作者：
A. Banerjee
编码来自新泽西水泡性口炎病毒血清型的整个糖蛋白的 cDNA 克隆的核苷酸序列
影响因子：
5.4
作者：
C. Gallione;J. Rose
通讯作者：
J. Rose