Southeast Asian ovalocytic (SAO) erythrocytes have a cold sensitive cation leak: implications for in vitro studies on stored SAO red cells
Southeast Asian ovalocytic (SAO) erythrocytes have a cold sensitive cation leak: implications for in vitro studies on stored SAO red cells
复制标题
DOI：
10.1016/s0005-2736(98)00231-4
复制
发表时间：
1999-01-12
影响因子：
3.4
通讯作者：
Tanner, MJA
中科院分区：
文献类型：
--
作者：
Bruce, LJ;Ring, SM;Tanner, MJA
东南亚卵形红细胞增多症 (SAO) 是由异常带 3 的杂合性存在引起的，这会导致红细胞特性发生多种改变。尽管早期研究表明，SAO 红细胞在体外难以抵抗疟原虫恶性疟原虫的侵袭。最近的一项研究搁置了新鲜的 SAO 细胞会受到寄生虫的侵袭，但在储存过程中会抵抗侵袭，因为细胞内 ATP 的消耗速度比正常情况更快。在这里，我们发现，在低温保存时，SAO 红细胞比正常红细胞更容易渗漏钠和钾。当细胞储存在 25 或 37°C 时，这种泄漏不太明显。在 37°C 冷藏的 SAO 红细胞中孵育 3.5 小时并未将钠和钾恢复到正常水平，可能是因为在 37°C 下孵育，冷藏的 SAO 红细胞中耗尽的 ATP 水平进一步降低：SAO 红细胞的泄漏增加是非特异性的，并延伸到钙离子， 牛磺酸、甘露醇和蔗糖。这些结果表明 SAO 红细胞在冷却时会发生结构变化。由于许多描述 SAO 红细胞特性改变的报告都使用了冷藏保存的细胞，因此需要使用从未冷冻的 SAO 红细胞重新评估这些结果，以评估细胞在体内条件下是否具有相同的异常特性。 (C) 1999 Elsevier Science B.V. 保留所有权利。
South-east Asian ovalocytosis (SAO) results from the heterozygous presence of an abnormal band 3, which causes several alterations in the properties of the erythrocytes. Although earlier studies suggested that SAO crythrocytes are refractory to invasion in vitro by the malarial parasite Plasmodium falciparum. a more recent study shelved that fresh SAO cells were invaded by the parasites, but became resistant to invasion on storage because intracellular ATP was depleted more rapidly than normal. Here we show that SAO red cells an much more leaky to sodium and potassium than normal red cells when stored in the cold. This leak was much less marked when the cells were stored at 25 or 37 degrees C. Incubation for 3.5 h at 37 degrees C of cold-stored SAO red cells did not restore sodium and potassium to normal levels, probably because the depleted ATP level in cold-stored SAO red cells is further reduced with incubation at 37 degrees C: The increased leakiness of SAO red cells is non-specific and extends to calcium ions, taurine, mannitol and sucrose. These results suggest that SAO red cells undergo a structural change on cooling. Since many of the reports describing altered properties of SAO red cells have used cells which have been stored in the cold, these results need re-evaluation using never-chilled SAO red cells to assess whether the cells have the same abnormal properties under in vivo conditions. (C) 1999 Elsevier Science B.V. All rights reserved.