Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies.
Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies.
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DOI：
10.1364/optica.4.000222
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发表时间：
2017
期刊：
影响因子：
10.4
通讯作者：
Yun SH
中科院分区：
文献类型：
--
作者：
Humar M;Yun SH
荧光和生物发光被广泛用于从分子到整个生物体水平研究生物系统。然而，它们的宽带发射通常是灵敏光谱测量和复用的瓶颈。为了克服这一限制，发射器可以与光学腔模耦合以产生窄带光谱特征。在这里，我们展示了几种类型的发射器谐振器复合物的荧光或生物发光蛋白质和人工或自然形成的光学谐振器。我们设计细胞以表达与ABHD 5融合的绿色荧光蛋白（GFP），ABHD 5与支持回音壁模式（WGM）的油或脂滴结合。基因整合的复合物具有明确定义的WGM光谱峰。在有丝分裂过程中，我们测量了GFP包被的BaTiO 3珠（直径2.56 μm）的WGM峰。最后，我们证明了腔增强生物发光使用包被的珠和生化激发。通过生物系统内部的腔共振来定制自发发射的能力应该在生物传感、成像和细胞标记中具有应用。
Fluorescence and bioluminescence are widely used to study biological systems from molecular to whole organism level. However, their broadband emission is often a bottleneck for sensitive spectral measurements and multiplexing. To overcome the limitation, the emitters can be coupled with optical cavity modes to generate narrowband spectral features. Here we demonstrate several types of emitter-resonator complexes made of fluorescent or bioluminescent proteins and artificially or naturally formed optical resonators. We engineered cells to express green fluorescent protein (GFP) fused with ABHD5, which binds to oil or lipid droplets supporting whispering gallery modes (WGM). The genetically-integrated complexes feature well-defined WGM spectral peaks. We measured WGM peaks from GFP-coated BaTiO3 beads (2.56 μm in diameter) during mitosis. Finally, we demonstrate cavity-enhanced bioluminescence using luciferase-coated beads and biochemical excitation. The ability to tailor spontaneous emission by cavity resonance inside biological systems should have applications in biological sensing, imaging and cell tagging.