Latent membrane protein-1 oncogene deletions in nasopharyngeal carcinoma in Caucasian patients
Latent membrane protein-1 oncogene deletions in nasopharyngeal carcinoma in Caucasian patients
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DOI：
10.1080/00016480310000593
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发表时间：
2003-06-01
影响因子：
1.4
通讯作者：
Bellas, C
中科院分区：
文献类型：
--
作者：
Plaza, G;Santón, A;Bellas, C
目的：潜伏膜蛋白-1(LMP-1)是一种EB病毒(EBV)转化蛋白，在鼻咽癌中表达。在亚洲鼻咽癌患者中发现了LMP-1癌基因30个碱基的缺失。这项研究的目的是评估鼻咽癌与高加索患者中EBV缺失的相关性。材料和方法--选择了27例确诊为鼻咽癌的患者。使用国际抗癌联合会系统，大多数国家癌症被归类为III和IV期。在这些病例中发现了福尔马林固定的、石蜡包埋的鼻咽癌标本。复习苏木精-伊红切片，采用LOG-RANK方法进行生存分析。对EBV编码的非多聚腺化RNA进行原位杂交，用免疫组织化学方法检测LMP-1的表达。用聚合酶链式反应检测鼻咽癌组织和EBV相关对照组织中LMP-1癌基因的缺失情况。结果：鼻咽癌组织和对照组织中LMP-1基因缺失的发生率分别为67%和30%，差异有统计学意义(p=0.01，x~2检验)。LMP-1缺失与鼻咽癌患者的预后无关(野生型LMP-1株的5年生存率为33%，缺失型LMP-1株的5年生存率为24%；p=0.053，对数等级检验)。结论：超过一半的高加索鼻咽癌患者存在LMP-1癌基因30bp的缺失。携带LMP-1癌基因部分缺失的EB病毒可能在高加索人鼻咽癌的发病中起一定作用。
Objective-The latent membrane protein-1 (LMP-1) is an Epstein-Barr virus (EBV)-transforming protein expressed in nasopharyngeal carcinoma (NPC). A 30-bp deletion in the LMP-1 oncogene has been described in NPC patients from Asia. The purpose of this study was to evaluate the association between NPC and such an EBV deletion in Caucasian patients.Material and Methods-Twenty-seven patients with a diagnosis of NPC were selected. Most of the NPCs were classified as Stages III and IV using the International Union Against Cancer system. Formalin-fixed, paraffin-embedded NPC specimens were found for these cases. Hematoxylin-eosin slides were reviewed and survival analysis was done using the log-rank method. In situ hybridization for EBV-encoded non-polyadenylated RNAs and expression of LMP-1 by means of immunohistochemistry was also performed. Polymerase chain reaction for LMP-1 oncogene analysis was performed to detect the presence of a 30-bp deletion in NPC specimens and EBV-related controls.Results-The 30-bp deletion was identified in 67% of NPC cases and in 30% of controls, a statistically significant difference (p = 0.01, chi(2) test). LMP-1 deletion was not statistically associated with a worse prognosis in NPC patients (5-year survival: 33% in wild-LMP-1 strains vs 24% in deleted-LMP-1 strains; p = 0.053, log-rank test).Conclusion-A 30-bp deletion in the LMP-1 oncogene is present in more than half of Caucasian NPC cases. EBV carrying partial deletions in the LMP-1 oncogene may play a role in the pathogenesis of NPC in Caucasian patients.