VASOACTIVE-INTESTINAL-PEPTIDE STIMULATES ACTH AND CORTICOSTERONE RELEASE AFTER INJECTION INTO THE PVN
VASOACTIVE-INTESTINAL-PEPTIDE STIMULATES ACTH AND CORTICOSTERONE RELEASE AFTER INJECTION INTO THE PVN
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DOI：
10.1016/0167-0115(94)90068-x
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发表时间：
1994-05-26
影响因子：
--
通讯作者：
SANDER, LD
中科院分区：
文献类型：
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作者：
ALEXANDER, LD;SANDER, LD
血管活性肠肽（Vasoactive intestinal peptide， VIP）是一种原产于肠道的神经肽，广泛分布于中枢和周围神经系统，具有广泛的生物学作用。在本研究中，研究了VIP对空腹、自由活动的雄性大鼠血浆ACTH和皮质酮（CORT）分泌的影响。雄性大鼠长期植入下丘脑室旁核（PVN）插管，注射VIP剂量（0.15 ~ 3.0 nmol/大鼠）或生理盐水（对照组）。立即在给药前和给药后15、30、60、90和120分钟从静脉导管中采集血样（0.6 ml）。PVN给药VIP可使血浆ACTH和CORT水平呈剂量依赖性增加，且在给药后15 min达到最大效果。在测试的最高剂量下，VIP使ACTH和CORT分别增加到时间匹配对照组的167%和342%。这些结果表明PVN是VIP诱导血浆ACTH和CORT升高的敏感部位，并暗示先前在该区域发现的VIP结合位点和免疫反应终端可能参与垂体-肾上腺轴的中枢调节。
Vasoactive intestinal peptide (VIP), a neuropeptide originally isolated from the intestine, is widely distributed in the central and peripheral nervous systems and exhibits a broad range of biological actions. In the present study the effects of VIP on plasma ACTH and corticosterone (CORT) secretion were investigated in fasted, freely-moving male rats. Male rats, chronically implanted with a hypothalamic paraventricular nucleus (PVN) cannula, were injected with VIP doses (0.15-3.0 nmol/rat) of VIP or saline (control). Blood samples were collected (0.6 ml) from an intravenous catheter immediately preceding and at 15, 30, 60, 90 and 120 min following peptide administration. PVN administration of VIP increased the plasma ACTH and CORT levels in a dose-dependent manner and the maximal effect was obtained at 15 min after administration. At the highest dose tested, VIP increased ACTH and CORT to 167% and 342% of time-matched controls, respectively. These results demonstrate that the PVN is a sensitive site for VIP-induced elevation of plasma ACTH and CORT and imply that the VIP binding sites and immunoreactive terminals previously identified in this region may be involved in the central regulation of the pituitary-adrenal axis.