Prostaglandin endoperoxide-H synthase-1 and -2 messenger ribonucleic acid levels in human amnion with spontaneous labor onset.
Prostaglandin endoperoxide-H synthase-1 and -2 messenger ribonucleic acid levels in human amnion with spontaneous labor onset.
复制标题
自然分娩时人羊膜中前列腺素内过氧化物-H合酶1和-2信使核糖核酸水平。
DOI：
10.1210/jcem.80.2.7852513
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
David M. Olson
中科院分区：
文献类型：
--
作者：
Jonathan J. Hirst;F. Teixeira;T. Zakar;David M. Olson
子宫腔内前列腺素（PG）的产生增加在导致妇女分娩开始的过程中起着关键作用。两个PG内过氧化物-H合酶（PGHS）同工酶已被确定在一些细胞类型。PGHS-1在大多数情况下组成型表达，而PGHS-2的表达被几种激动剂快速诱导。本研究的目的是确定PGHS-1和PGHS-2的表达水平之前和之后的自然分娩（SL）在羊膜和评估PGHS-1和PGHS-2的酶活性的贡献。我们建立并验证了核糖核酸酶保护测定法，以定量羊水中PGHS-1和PGHS-2信使核糖核酸（mRNA）水平。PGHS酶活性用已建立的测定法测量。使用人PGHS-1和PGHS-2互补DNA产生用于保护测定的反义RNA探针。这些探针在北方印迹上特异性地检测羊膜RNA样品中PGHS-1的2.8-脱氢酶mRNA和PGHS-2的4.8-脱氢酶mRNA。我们测量了足月SL后患者和足月择期剖宫产（CS）未临产患者羊膜中mRNA水平。SL后PGHS-2 mRNA水平明显高于CS羊膜[分别为5.18 +/- 1.08（n = 16）和2.27 +/- 0.50（n = 15），密度单位; P < 0.02]，而分娩后PGHS-1 mRNA水平与CS样品相比无差异。25例患者PGHS-2 mRNA水平与PGHS酶活性呈正相关（r = 0.65-0.88; P < 0.05）。PGHS-1 mRNA水平与酶活性之间无相关性。我们得出结论，PGHS-2 mRNA存在于人羊膜中，其水平升高SL发病后，它们与酶活性。在分娩开始时PGHS活性的刺激可能涉及PGHS-2表达的增加。PGHS-1在人羊膜中的表达与分娩无关。
Increased prostaglandin (PG) production within the uterine compartment has a pivotal role in the processes leading to labor onset in women. Two PG endoperoxide-H synthase (PGHS) isoenzymes have been identified in a number of cell types. PGHS-1 is constitutively expressed in most cases, whereas PGHS-2 expression is rapidly induced by several agonists. The aims of this study were to determine the levels of PGHS-1 and PGHS-2 expression before and after spontaneous labor (SL) onset in the amnion and to assess the contribution of PGHS-1 and PGHS-2 to enzyme activity. We established and validated ribonuclease protection assays to quantify PGHS-1 and PGHS-2 messenger ribonucleic acid (mRNA) levels in the amnion. PGHS enzyme activity was measured with an established assay. The antisense RNA probes used in the protection assays were generated using human PGHS-1 and PGHS-2 complementary DNAs. These probes specifically detected the 2.8-kilobase mRNA of PGHS-1 and the 4.8-kilobase mRNA of PGHS-2 in amnion RNA samples on Northern blots. We measured mRNA levels in amnion from patients after SL at term and from patients not in labor undergoing elective cesarean section (CS) at term. PGHS-2 mRNA levels were markedly higher after SL compared to levels in CS amnion [5.18 +/- 1.08 (n = 16) and 2.27 +/- 0.50 (n = 15), densitometric units, respectively; P < 0.02], whereas there was no difference in PGHS-1 mRNA levels after labor compared with CS samples. PGHS-2 mRNA levels were also positively correlated with PGHS enzyme activity in 4 separate assays with a total of 25 patients (r = 0.65-0.88; P < 0.05). There was no correlation between PGHS-1 mRNA levels and enzyme activity. We conclude that PGHS-2 mRNA is present in human amnion; its levels are elevated after SL onset, and they are correlated with enzyme activity. The stimulation of PGHS activity at labor onset probably involves increased expression of PGHS-2. The expression of PGHS-1 does not change in association with labor in human amnion.
小鼠诱导型和组成型前列腺素合酶/环氧合酶基因的染色体组织。
影响因子：
4.4
作者：
Wen,PZ;Warden,C;Fletcher,BS;Kujubu,DA;Herschman,HR;Lusis,AJ
通讯作者：
Lusis,AJ
妊娠晚期绵羊子叶组织中前列腺素内过氧化物合酶亚型2的诱导。
影响因子：
4.8
作者：
Wimsatt,J;Nathanielsz,PW;Sirois,J
通讯作者：
Sirois,J