Integrins and syndecan-4 make distinct, but critical, contributions to adhesion contact formation
Integrins and syndecan-4 make distinct, but critical, contributions to adhesion contact formation
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DOI：
10.1039/b614610d
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发表时间：
2007-01-01
期刊：
影响因子：
3.4
通讯作者：
Humphries, Martin J.
中科院分区：
文献类型：
--
作者：
Bass, Mark D.;Morgan, Mark R.;Humphries, Martin J.
在细胞粘附到纤连蛋白的过程中，存在肌动蛋白细胞骨架的主要重组和伴随的粘附复合物的形成。粘附受体的筛选研究报道，整合素单独或整合素和多配体蛋白聚糖-4两者介导含粘着斑蛋白的粘附的形成，并且这些研究中的差异归因于所用配体的密度和构象完整性。我们已经努力通过ELISA分析固定化多肽来解决这些问题，并且发现在折叠配体的等密度条件下，整合素α（5）β（1）和多配体蛋白聚糖-4的配体对于粘着斑形成是必需的。我们还表明，整合素和syndecan-4发挥相当不同的作用，在粘附接触成熟和不可互换。这些结果有助于我们了解细胞如何有效地响应基质环境的变化，这应该证明有助于开发帮助伤口愈合的方法。
During cell adhesion to fibronectin there is a major reorganisation of the actin cytoskeleton and concomitant formation of adhesion complexes. Conflicting studies of adhesion receptors report that either integrin alone, or both integrin and syndecan-4 mediate the formation of vinculin-containing adhesions, and differences in these studies have been attributed to the density and conformational integrity of ligands used. We have endeavoured to resolve these issues by ELISA analysis of immobilised polypeptides, and found that ligands of both integrin alpha(5)beta(1) and syndecan-4 are necessary for focal adhesion formation under conditions of equivalent density of folded ligand. We also demonstrate that integrin and syndecan-4 play quite distinct roles in adhesion contact maturation and are not interchangeable. These results help us to understand how cells respond efficiently to changes in matrix environment, which should prove useful for developing approaches to aid wound healing.