ABSENCE OF THE COOH-TERMINAL PEPTIDE ON ECTOPIC HUMAN CHORIONIC GONADOTROPIN B-SUBUNIT (hCGB)
ABSENCE OF THE COOH-TERMINAL PEPTIDE ON ECTOPIC HUMAN CHORIONIC GONADOTROPIN B-SUBUNIT (hCGB)
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异位人绒毛膜促性腺激素 B 亚基 (hCGB) 上 COOH 末端肽的缺失
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
R. Pattillo
中科院分区：
文献类型：
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作者：
L. Cole;S. Birken;S. Sutphen;R. Hussa;R. Pattillo
比较体外培养的宫颈癌细胞异位hCGB和标准hCGB的物理性质和免疫反应性。通过离子交换色谱法检查制剂的异质性，并通过分析凝胶过滤法检查分子大小。鉴定了两种不同形式的异位hCGB。一种形式与标准hCGB无法区分，而另一种虽然更大，但缺乏特征性的COOH末端肽（CTP）。这通过CTP上的决定簇的特异性抗血清识别该分子的失败以及通过通常在该区域中发现的0-1连接的寡糖和嗜热菌蛋白酶切割位点的明显缺失来显示。
A comparison of the physical properties and immunoreactivity of ectopic hCGB from cultured cervical cancer cells and standard hCGB was performed. The heterogeneity of the preparations was examined by ion-exchange chromatography, and the molecular size by analytical gel filtration. Two distinct forms of ectopic hCGB were identified. One form was indistinguishable from standard hCGB, while the other, although larger, lacked the characteristic COOH-terminal peptide (CTP). This was shown by the failure of antisera specific for determinants on the CTP to recognize this molecule, and by the apparent absence of the 0-1 inked oligosaccharides and thermolysin cleavage site normally found in this region.