Lotus burttii takes a position of the third corner in the Lotus molecular genetics triangle
Lotus burttii takes a position of the third corner in the Lotus molecular genetics triangle
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DOI：
10.1093/dnares/12.1.69
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发表时间：
2005-02-28
期刊：
影响因子：
4.1
通讯作者：
Grant, WF
中科院分区：
文献类型：
--
作者：
Kawaguchi, M;Pedrosa-Harand, A;Grant, WF
为了进一步巩固百脉根的分子遗传体系，了解百脉根的生物多样性，本研究以来自巴基斯坦西部的百脉根B-303作为岐阜生态型（B-129-S9）的第三个杂交组合，对其进行遗传分析。L. Burttii是一种相对较小且开花较早的植物，具有不破碎的特性。荧光原位杂交（FISH）结果表明，该菌1号染色体短臂与岐阜菌相似。burttii与岐阜相似，表明在L. Miyakojima MG-20，不存在于L。伯蒂。此外，L. burttii与Gifu和MG-20相比具有更高水平的DNA多态性，使得能够设计共显性标记如SSR、CAPS和dCAPS。利用岐阜和L. burttii，在易位位点共分离的共显性标记可以扩大。为了使遗传背景标准化，L。Burttii自交9代，L.建立了Burttii B-303-S9菌株。
In order to consolidate molecular genetic system in Lotus japonicus and to further access the biological diversity in Lotea, we introduce here Lotus burttii B-303 derived from West Pakistan as the third crossing partner of the Gifu ecotype (B-129-S9) for a genetic analysis. L. burttii is a relatively small and early flowering plant with non-shattering behavior. The general chromosome morphology is very similar to Gifu, and fluorescence in situ hybridization (FISH) analysis revealed that the short arm of chromosome 1 in L. burttii is comparable to that of Gifu, indicating that the translocation event involving chromosomes 1 and 2, which was observed in L. japonicus Miyakojima MG-20, is not present in L. burttii. In addition L. burttii has a higher level of DNA polymorphism compared to Gifu and MG-20 enabling design of codominant markers such as SSR, CAPS and dCAPS. Using an F-2 Population from a cross between Gifu and L. burttii, codominant makers that co-segregated at the translocation site could be expanded. In order to normalize the genetic background, L. burttii was inbred for nine generations and the germplasm. L. burttii B-303-S9 was established.