Ethylene is involved in the nodulation phenotype of Pisum sativum R50 (sym 16), a pleiotropic mutant that nodulates poorly and has pale green leaves
Ethylene is involved in the nodulation phenotype of Pisum sativum R50 (sym 16), a pleiotropic mutant that nodulates poorly and has pale green leaves
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DOI：
10.1093/jexbot/51.346.885
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发表时间：
2000-05-01
影响因子：
6.9
通讯作者：
Sloetjes, LL
中科院分区：
文献类型：
--
作者：
Guinel, FC;Sloetjes, LL
R50的特征是一个多效性豌豆突变体;它形成很少的根瘤，侧根短，身材矮小，叶浅。利用嫁接技术，R50苍白被发现控制的突变体的芽，而其根调节的再生表型。R50的苍白是由于其幼叶中的总叶绿素含量低于正常水平。这种缺陷似乎可以随着年龄的增长而克服，因为随着植物的成熟，老叶的叶绿素水平会增加。身材的减少归因于较短的节间，最老的节间比亲本Sparkle的节间厚。在根瘤菌接种后，R50形成与Sparkle一样多的感染线。然而，大多数这些都是在内皮层被捕。螺纹似乎已经失去了他们的方向增长中柱，他们盘绕在扩大皮层细胞。此外，很少有感染线与内皮层细胞的分裂有关。这些败育的根瘤原基是异常的、扁平的，主要由垂周分裂的细胞组成。通过用乙烯抑制剂处理根来恢复R50的结瘤。R50突变体进一步支持乙烯在调节根瘤菌感染中的假定作用，其可能在控制原基发育中起作用。
R50 is characterized as a pleiotropic pea mutant; it forms few nodules and has short lateral roots, short stature and pale leaves. Using grafting techniques, R50 paleness was found to be controlled by the shoot of the mutant whereas the nodulation phenotype was regulated by its root. The paleness of R50 is due to a lower than normal total chlorophyll content in its young leaves. The defect appears to be overcome with age because, as the plant matures, the chlorophyll levels increase in the older leaves. The reduction in stature is attributed to shorter internodes, and the oldest internodes are thicker than those of the parent Sparkle. Upon rhizobial inoculation, R50 forms as many infection threads as Sparkle. However, most of these are arrested in the inner cortex. The threads appear to have lost their directional growth towards the stele, and they coil around within enlarged cortical cells. In addition, very few infection threads are associated with divisions of the inner cortical cells. These aborted nodule primordia are abnormal, flat and mainly composed of cells which have divided anticlinally only. Nodulation of R50 was restored by treating the roots with ethylene inhibitors. The R50 mutant further supports the postulated role of ethylene in regulating rhizobial infection with a probable role in the control of the primordium development.