Reversible electrochemical detection of nonelectroactive polyions
Reversible electrochemical detection of nonelectroactive polyions
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DOI：
10.1021/ja037167n
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发表时间：
2003-09-17
影响因子：
15
通讯作者：
Bakker, E
中科院分区：
文献类型：
--
作者：
Shvarev, A;Bakker, E
选择性萃取原理用于识别肝素和鱼精蛋白等非电活性多离子，但高离子价态使萃取过程不可逆。本文提出了此类多离子的可逆检测的响应原理。多离子分析物到膜的提取及其随后的反提取现在由电化学控制。该方法以鱼精蛋白为电极，具有良好的稳定性和重现性。该方法对设计多离子分析物的化学识别原理具有重要意义。
Selective extraction principles for the recognition of nonelectroactive polyions such as heparin and protamine exist, but the high ionic valency renders the extraction process irreversible. A response principle for the reversible detection of such polyions is proposed here. The extraction of the polyionic analyte to the membrane and its subsequent back-extraction is now controlled electrochemically. The principle is established with a protamine electrode, and excellent stability and reproducibility are demonstrated. This method has important implications for the design of chemical recognition principles for polyionic analytes.