Genetic interactions between naturally occurring alleles at quantitative trait loci and mutant alleles at candidate loci affecting bristle number in Drosophila melanogaster.
Genetic interactions between naturally occurring alleles at quantitative trait loci and mutant alleles at candidate loci affecting bristle number in Drosophila melanogaster.
复制标题
数量性状基因座上自然发生的等位基因与候选基因座上影响果蝇刚毛数量的突变等位基因之间的遗传相互作用。
DOI：
10.1093/genetics/144.4.1497
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Langley,CH
中科院分区：
文献类型：
--
作者：
Long,AD;Mullaney,SL;Mackay,TF;Langley,CH
在此之前，我们映射数量性状位点（QTL）影响短期选择腹部刚毛数量的7个暗示性区域，包含涉及刚毛发育和/或有成年刚毛数量突变表型的位点，因此在自然种群中的刚毛数量QTL的候选人。为了验证这一假设，即选择反应的因素与这些候选基因座遗传相互作用，选择系的高和低染色体与候选基因座上含有野生型或突变等位基因的染色体杂交，并记录杂合子内的刚毛数量。通过方差分析检测为显著的选择系 * 交叉效应的互补的定量失败可以解释为所选染色体上的因子与候选基因座之间的等位性或上位性的证据。在一些候选基因座（bb、emc、h、Dl、Hairless）处的突变显示出与所选染色体的强相互作用，而其它基因座（ASC、abd、Scr）弱相互作用或根本不相互作用（N、mab、E（spl））。这些结果支持了这一假设，即一些候选位点，最初确定通过突变的刚毛数量的大的影响，要么港口或接近成员在同一遗传途径的变异，有助于直立变异刚毛数量。
Previously, we mapped quantitative trait loci (QTL) affecting response to short-term selection for abdominal bristle number to seven suggestive regions that contain loci involved in bristle development and/or that have adult bristle number mutant phenotypes, and are thus candidates for bristle number QTL in natural populations. To test the hypothesis that the factors contributing to selection response genetically interact with these candidate loci, high and low chromosomes from selection lines were crossed to chromosomes containing wild-type or mutant alleles at the candidate loci, and the numbers of bristles were recorded intransheterozygotes. Quantitative failure to complement, detected as a significant selection line*cross effect by analysis of variance, can be interpreted as evidence for allelism or epistasis between the factors on selected chromosomes and the candidate loci. Mutations at some candidate loci (bb, emc, h, Dl, Hairless) showed strong interactions with selected chromosomes, whereas others interacted weakly (ASC, abd, Scr) or not at all (N, mab, E(spl)). These results support the hypothesis that some candidate loci, initially identified through mutations of large effect on bristle number, either harbor or are close members in the same genetic pathway as variants that contribute to standing variation in bristle number.