Detection of Helicobacter pylori DNA in fecal samples from infected individuals
Detection of Helicobacter pylori DNA in fecal samples from infected individuals
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DOI：
10.1128/jcm.37.7.2236-2240.1999
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发表时间：
1999-07-01
影响因子：
9.4
通讯作者：
Powell, SM
中科院分区：
文献类型：
--
作者：
Gramley, WA;Asghar, A;Powell, SM
从22名受试者中采集粪便、胃活检和血清样本。从粪便中提取DNA，扩增，并与幽门螺杆菌16S rRNA基因特异性引物杂交。类似地分析来自胃活检标本的DNA以进行比较。通用引物用于确认从样品中成功提取DNA。以盲法对组织学、血清学和DNA分析进行评分。通用引物扩增验证了从所有粪便和胃组织标本中成功提取DNA。胃组织DNA检测为H. pylori感染，与组织学和血清学结果完全相关。粪便DNA为H. pylori的分子检测结果显示，这11例H.幽门阳性的受试者。组织学、血清学和胃组织DNA分析阴性的所有受试者粪便DNA分析也为阴性。与组织学、血清学和胃组织DNA分析相比，我们的粪便DNA检测的敏感性为73%，特异性为100%。
Stool, gastric biopsy, and serum samples were collected from 22 subjects. DNA from stool was extracted, amplified, and hybridized with primers specific for the 16S rRNA gene of Helicobacter pylori. DNA from gastric biopsy specimens was analyzed similarly for comparison. Universal primers were used to confirm successful extraction of DNA from samples. Histologic, serologic, and DNA analyses were scored in a blinded fashion. Universal primer amplification verified successful DNA extraction from all stool and gastric tissue specimens. The gastric tissue DNA assay was positive for H. pylori in 11 of the 22 subjects, correlating completely with histologic and serologic results. Stool DNA was positive for H. pylori by our molecular assay in 8 of these 11 H. pylori-positive subjects. All subjects who were negative by histologic, serologic, and gastric tissue DNA analyses were also negative by stool DNA analysis. Compared to histology, serology, and gastric tissue DNA analyses, the sensitivity of our stool DNA assay was 73%, with a specificity of 100%.