STUDIES ON PIG LIVER MICROSOMES .1. ENZYMIC AND PIGMENT COMPOSITION OF DIFFERENT MICROSOMAL FRACTIONS
STUDIES ON PIG LIVER MICROSOMES .1. ENZYMIC AND PIGMENT COMPOSITION OF DIFFERENT MICROSOMAL FRACTIONS
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DOI：
10.1016/0003-9861(58)90095-x
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发表时间：
1958-01-01
影响因子：
3.9
通讯作者：
GARFINKEL, D
中科院分区：
文献类型：
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作者：
GARFINKEL, D
描述了猪肝微粒体的轻、重分离。轻微粒体富含细胞色素b5，在电镜下显示为表面光滑的囊泡；此外，这些表面光滑的微粒体在生物化学和形态上与表面粗糙的微粒体不同。描述了增溶剂和低pH值对微粒体的影响，血红蛋白的结合，可能的季节性影响，以及微粒体色素，包括当与CO还原复合物时在450 m[mu]处显示吸收峰的影响。描述了一种微粒体物质（可能是分解产物），它能迅速氧化细胞色素b5，并讨论了其可能的意义。
A light-heavy fractionation of pig liver microsomes is described. The light microsomes are rich in cytochrome b5 and evidence is presented that these are the microsomes appearing as smooth-surfaced vesicles in the electron microscope; also that these smooth-surfaced microsomes are biochemically and morphologically distinct from rough-surfaced microsomes. Descriptions are given of effects of solubilizing agents and low pH on microsomes, of the binding of hemoglobin, of possible seasonal effects, and of microsomal pigments, including one which when reduced complexes with CO to show an absorption peak at 450 m[mu]. A microsomal substance (probably a breakdown product) which rapidly oxidizes cytochrome b5 is described, and its possible significance is discussed.