Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles.
Characterization of Innate Immune Responses of Human Endothelial Cells Induced by Porphyromonas gingivalis and Their Derived Outer Membrane Vesicles.
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DOI：
10.3389/fcimb.2016.00139
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发表时间：
2016
影响因子：
5.7
通讯作者：
Xie H
中科院分区：
文献类型：
--
作者：
Ho MH;Guo ZM;Chunga J;Goodwin JS;Xie H
动脉粥样硬化是一种慢性血管炎性疾病，是世界范围内发病率和死亡率最常见的原因之一。牙龈卟啉单胞菌在动脉粥样硬化中的作用得到了流行病学、临床、免疫学和分子研究的支持。以前我们报道过牙龈卟啉单胞菌囊泡比其原始细胞具有更高的侵袭效率。在这里，我们进一步比较牙龈卟啉单胞菌细胞及其囊泡在表达趋化蛋白，包括CXCL 1，CXCL 2和CXCL 8，和粘附分子，如E-选择素在人脐静脉内皮细胞（HUVECs）的作用。牙龈卟啉单胞菌33277细胞和囊泡都能够上调这些分子的表达，而囊泡作为与动脉粥样硬化发展相关的炎症反应的更有效的诱导剂，从而导致显著的单核细胞粘附到单层HUVEC。有趣的是，我们发现牙龈卟啉单胞菌诱导的内皮细胞中CXCL 8和E-选择素的表达升高与牙龈卟啉单胞菌细胞和囊泡的侵袭能力相关。非侵入性细菌细胞和囊泡对这些基因的表达没有影响。这项研究强调了牙龈卟啉单胞菌细胞和囊泡在动脉粥样硬化起始中的潜在风险，并为开发针对细菌相关动脉粥样硬化的新疗法提供了潜在靶点。
Atherosclerosis, a chronic inflammatory disease of the blood vessels, is one of the most common causes of morbidity and mortality world-wide. Involvement of Porphyromonas gingivalis in atherosclerosis is supported by observations from epidemiological, clinical, immunological, and molecular studies. Previously we reported that P. gingivalis vesicles have a much higher invasive efficiency than their originating cells. Here, we further compare the role of P. gingivalis cells and their vesicles in expression of chemoattractant proteins including CXCL1, CXCL2, and CXCL8, and adhesive molecules such as E-selectin in human umbilical vein endothelial cells (HUVECs). Both P. gingivalis 33277 cells and vesicles were able to up-regulate expression of these molecules, while the vesicles acted as more potent inducers of the inflammatory response associated with the development of atherosclerosis, consequently resulting in significant monocyte adhesion to a monolayer of HUVECs. Interestingly, we found that elevated expression of CXCL8 and E-selectin in endothelial cells induced by P. gingivalis correlated with the invasive ability of P. gingivalis cells and vesicles. Non-invasive bacterial cells and vesicles had no effect on expression of these genes. This study highlights the potential risk of P. gingivalis cells and vesicles in initiation of atherosclerosis and provides a potential target for the development of novel therapeutics against bacteria-associated atherosclerosis.
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Keystone-Pathogen假设。
DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Hutcherson JA;Bagaitkar J;Nagano K;Yoshimura F;Wang H;Scott DA
通讯作者：
Scott DA
影响因子：
11.8
作者：
Macia, Eric;Ehrlich, Marcelo;Kirchhausen, Tomas
通讯作者：
Kirchhausen, Tomas
影响因子：
3.1
作者：
Chou, HH;Yumoto, H;Genco, CA
通讯作者：
Genco, CA
影响因子：
3.4
作者：
Reife, RA;Coats, SR;Darveau, RP
通讯作者：
Darveau, RP