Broad-spectrum antifungal metabolites produced by the soil bacterium Serratia plymuthica A 153
Broad-spectrum antifungal metabolites produced by the soil bacterium Serratia plymuthica A 153
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DOI：
10.1016/j.soilbio.2003.12.008
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发表时间：
2004-04-01
影响因子：
9.7
通讯作者：
Welch, CJ
中科院分区：
文献类型：
--
作者：
Levenfors, JJ;Hedman, R;Welch, CJ
氯化大环内酯类化合物NA、B和NE是由土壤细菌沙雷氏菌产生的。Haterumalide NA、B和NE在0.5µg·ml~(-1)浓度下可完全抑制核盘菌菌核的具鞘形成。该菌的子囊孢子萌发在0.8~3.0µg·ml~(-1)浓度范围内受到抑制。Haterumalide NA、B和NE在0.4~40µg·ml~(-1)浓度范围内对其他几种丝状真菌和卵菌的孢子萌发有抑制作用，但对白色念珠菌无抑制作用。由于Haterumalide X迅速转化为Haterumalide NA，因此无法收集其抑制数据。该菌还产生了另外两种抗真菌代谢物：吡咯硝酸酯和1-乙酰基-7-氯-1-H-吲哚，与杂毛菌素不同，它们不抑制菌核上的具鞘形成。在0.06~50µg·ml~(-1)的浓度范围内，吡咯硝菊酯、百草枯内酯NA、B和NE能有效地抑制丝状真菌的孢子萌发，而1-乙酰基-7-氯-1-H-吲哚只有在50µg·ml~(-1)以上的浓度时才能抑制孢子萌发。完全抑制孢子萌发所需的各化合物的最小抑制浓度因所测试的真菌种类而异。(C)2004爱思唯尔有限公司。保留所有权利。
Chlorinated macrolides, haterumalide NA, B and NE, and a new haterumalide X, were produced by the soil bacterium Serratia plymuthica. Haterumalides NA, B and NE caused complete suppression of apothecial formation in sclerotia of Sclerotinia sclerotiorum at a concentration of 0.5 mug ml(-1). Ascospore germination of this fungus was inhibited in the concentration range 0.8-3.0 mug ml(-1). Haterumalides NA, B and NE prevented spore germination of several other filamentous fungi as well as Oomycetes at concentrations ranging from 0.4 to 40 mug ml(-1), but did not show any effect against the yeast Candida albicans. Inhibition data could not be collected for haterumalide X due to its rapid conversion to haterumalide NA. The bacterium also produced two other antifungal metabolites: pyrrolnitrin and 1-acetyl-7-chloro-1-H-indole, which in contrast to the haterumalides, did not inhibit the apothecial formation on sclerotia. Pyrrolnitrin, and haterumalide NA, B and NE effectively inhibited spore germination of tested filamentous fungi at concentrations ranging from 0.06 to 50 mug ml(-1), whereas 1-acetyl-7-chloro-1-H-indole inhibited spore germination only at concentrations above 50 mug ml(-1). The minimal inhibitory concentrations of the respective compounds needed for total inhibition of spore germination varied for the fungal species tested. (C) 2004 Elsevier Ltd. All rights reserved.