Rapid metabolic adaptation in European sea bass (Dicentrarchus labrax) juveniles fed different carbohydrate sources after heat shock stress
Rapid metabolic adaptation in European sea bass (Dicentrarchus labrax) juveniles fed different carbohydrate sources after heat shock stress
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DOI：
10.1016/j.cbpa.2006.05.002
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发表时间：
2006-09-01
影响因子：
2.3
通讯作者：
Oliva-Teles, A.
中科院分区：
文献类型：
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作者：
Enes, P.;Panserat, S.;Oliva-Teles, A.
进行了一项研究，以评估两种膳食碳水化合物来源（蜡质玉米淀粉和葡萄糖）对海鲈鱼幼鱼（初始重量：24 g）对热休克处理（温度在24 h内从18 ℃升高至25 ℃）的代谢适应的影响。采用等氮、等脂日粮（含20%蜡质玉米淀粉（WS）和20%葡萄糖（GLU）），研究了玉米淀粉和葡萄糖对玉米蛋白质的影响。在两种温度（18 ℃和25 ℃）下将三组鱼喂食至接近饱足4周。然后，将先前保持在18摄氏度的鱼进行热休克（18摄氏度至25摄氏度），并在1周内继续用相同的饮食喂养。较高的水温显着提高生长性能，饲料效率，以及蛋白质效率比，独立的饮食。在25摄氏度，但不是在18摄氏度，鱼的增长喂养WS饮食高于鱼喂养的GLU饮食。血浆葡萄糖水平较高的鲈鱼喂养GLU饮食，不受水温的影响。喂食葡萄糖饮食或在高温（25摄氏度）下饲养的鱼与喂食淀粉饮食或在低温（18摄氏度）下饲养的鱼相比，显示出增强的肝脏糖酵解、脂肪生成和脂肪生成能力。对于大多数酶的研究，1周似乎是足够的时间代谢适应在鲈鱼提交了一个急性热shock.Regardless的碳水化合物源，HSP 70基因表达相似，在冷水（18摄氏度）和温水（25摄氏度）驯化鲈鱼。一个弱的下调后，观察到热休克只在鱼喂食GLU饮食。这表明HSP 70基因表达不受饲养温度本身的影响。(c)2006年爱思唯尔公司All rights reserved.
A study was conducted to evaluate the effect of two dietary carbohydrate sources (waxy maize starch and glucose) on the metabolic adaptation of sea bass juveniles (initial weight: 24 g) to a heat shock treatment (temperature rise from 18 degrees C to 25 degrees C within 24 h). Two isonitrogenous and isolipidic diets were fon-nulated to contain 20% waxy maize starch (WS diet) or 20% glucose (GLU diet). Triplicate groups of fish were fed to near satiation for 4 weeks at both temperatures (18 degrees C and 25 degrees C). Then, fish previously maintained at 18 degrees C were submitted to a heat shock (18 degrees C to 25 degrees C) and continued to be fed with the same diets during I more week. The higher water temperature significantly improved growth performance, feed efficiency, as well as protein efficiency ratio, independently of diet. At 25 degrees C, but not at 18 degrees C, growth of fish fed the WS diet was higher than that of fish fed the GLU diet. Plasma glucose levels were higher in sea bass fed the GLU diet and not influenced by water temperature. Fish fed a glucose diet or reared at high temperatures (25 degrees C) showed enhanced liver glycolytic, lipogenic and gluconeogenic capacities compared to fish fed a starch diet or reared at low temperatures (18 degrees C). For the majority of the enzymes studied, I week seemed to be enough time for metabolic adaptation in sea bass submitted to an acute heat shock.Irrespective of carbohydrate source, HSP70 gene expression was similar in both cold water (18 degrees C) and warm water (25 degrees C) acclimated sea bass. A weak down regulation was observed after heat shock only in fish fed the GLU diet. This suggests that HSP70 gene expression is not affected by the rearing temperature per se. (c) 2006 Elsevier Inc. All rights reserved.