An enzyme immunoassay to determine the levels of specific antibodies toward bacterial surface antigens in human immunoglobulin preparations and blood serum
An enzyme immunoassay to determine the levels of specific antibodies toward bacterial surface antigens in human immunoglobulin preparations and blood serum
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DOI：
10.1016/s0731-7085(99)00087-4
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发表时间：
1999-09-01
影响因子：
3.4
通讯作者：
Karamanos, NK
中科院分区：
文献类型：
--
作者：
Lamari, F;Anastassiou, ED;Karamanos, NK
人多价静脉免疫球蛋白(IVIG)制剂被用作重症监护病房(ICU)中严重脓毒症患者适当抗菌治疗的补充辅助手段，和/或作为免疫功能低下的宿主的预防性制剂，尤其是容易受到细菌感染的宿主。人们对静脉注射免疫球蛋白的有效性持怀疑态度，因为尚不清楚静脉注射免疫球蛋白是否通过提供足够数量的针对特定细菌病原体的特定抗体来确保体液免疫反应的增强。本文介绍了一种简便、重复性好的酶联免疫吸附试验，用于测定市售IVIG制剂中针对细菌表面抗原的特异性抗体的含量。该方法还可简便地用于血清中细菌抗体的测定。在两种IVIG(沙球蛋白(R)和伽马免疫(R))制剂中，对ICU中经常遇到的革兰氏阳性和阴性病原菌的特异性抗体水平进行了评估。关于某些临床细菌分离株的抗体含量，不仅在两个被测试的IVIG制剂之间，而且在每个IVIG制剂的不同批次之间都发现了显著的差异。来自同一批次的瓶子在两种制剂中没有显著差异(P小于或等于0.001)。IVIG制剂中特异性抗体水平的差异可能归因于供体池和制造程序之间的差异。对原发免疫缺陷患者的应用表明，高活性IVIG制剂的输注显著增强了他们对各种病原体的体液反应。本研究结果提示，IVIG制剂给药前病原体特异性抗体含量的测定对细菌感染的治疗具有重要意义。(C)1999 Elsevier Science B.V.保留所有权利。
Human polyvalent intravenous immunoglobulin (IVIG) preparations are used as a complementary aid to the proper antimicrobial treatment of severely septic patients in intensive care units (ICUs) and/or as a prophylactic agent to immunocompromized hosts, particularly prone to bacterial infections. There is skepticism about the usefulness of IVIGs since it is not known whether their administration ensures the enhancement of humoral immune responses by providing a sufficient amount of specific antibodies towards the specified bacterial pathogen to be treated. In this report, a simple and reproducible enzyme-linked immunosorbent assay for determining the content of specific antibodies against bacterial surface antigens in commercially available IVIG preparations is described. The method is also easily applied to determine the amount of bacterial antibodies in blood serum. The levels of specific antibodies toward Gram positive and negative pathogenic isolates often encountered in ICUs were estimated in two IVIG (Sandoglobulin(R) and Gamimmune(R)) preparations. Significant differences regarding the content of antibodies to certain clinically bacterial isolates were identified not only between the two IVIG preparations tested, but also among various lots from each IVIG preparation. No significant variation (P less than or equal to 0.001) among the bottles derived from the same lot was determined in both preparations. The variation in the levels of specific antibodies in IVIG preparations may be attributed to differences between the donor pools as well as the manufacturing procedure. Application of the method to patients with primary immune deficiencies showed that infusion of highly reactive IVIG preparations enhanced significantly their humoral response toward various pathogens. The results of this study suggest that the content determination of pathogen-specific antibodies in IVIG preparations before administration may be of great importance for treating bacterial infections. (C) 1999 Elsevier Science B.V. All rights reserved.