Unusual Selective Response to Glycoprotein over Sugar Facilitates Ultrafast Universal Fluorescent Immunoassay of Biomarkers
Unusual Selective Response to Glycoprotein over Sugar Facilitates Ultrafast Universal Fluorescent Immunoassay of Biomarkers
复制标题
对糖蛋白而非糖的异常选择性反应促进生物标志物的超快通用荧光免疫分析
DOI：
10.1021/acs.analchem.0c00403
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发表时间：
2020
影响因子：
7.4
通讯作者：
Wang Jian-Hua
中科院分区：
文献类型：
--
作者：
Wang Yi-Ting;Wu Na;Yang Ting;Wang Jian-Hua
酶联免疫吸附试验（ELISA）广泛应用于临床诊断。然而，传统的ELISA是劳动密集型的和冗长的。在本文中，基于抗体融合的硼酸修饰的氮化碳纳米片，证明了仅用20分钟的一步孵育的生物标志物的灵敏检测。用硼酸修饰氮化碳纳米片促进了在生理条件下的抗体结合，沿着荧光增强。靶抗原的存在导致荧光减弱，并确保一步免疫荧光检测。抗体/靶抗原的免疫识别与葡萄糖阻断的组合确保了生物标志物的高选择性测定。该协议是通过非糖蛋白，糖蛋白，和小分子毒素的目标的测定验证。通过同时测定人血清中的三重心脏生物标志物cTnI、Mb和CK-MB，证明了多重靶标检测能力。
The enzyme-linked immunosorbent assay (ELISA) is widely used in clinical diagnostics. However, conventional ELISA is labor-intensive and lengthy. Herein, the sensitive detection of biomarkers with only one-step incubation of 20 min is demonstrated, based on antibody-fused, boronic-acid-decorated carbon nitride nanosheets. The decoration of carbon nitride nanosheets with boronic acid facilitates antibody binding at physiological conditions along with a concomitant fluorescence enhancement. The presence of target antigen results in a decrement of the fluorescence and ensures one-step immunofluorescent detection. The immune recognition of the antibody/target antigen in combination with glucose blocking ensures a highly selective assay of the biomarkers. The protocol is validated by the assay of nonglycoprotein, glycoprotein, and small-molecular-toxin targets. The multiplex target detection capability is demonstrated by the simultaneous assay of the triple cardiac biomarker cTnI, Mb, and CK-MB in human serum.