Isolation and characterization of a new insect flavivirus from Aedes albopictus and Aedes flavopictus mosquitoes in Japan
Isolation and characterization of a new insect flavivirus from Aedes albopictus and Aedes flavopictus mosquitoes in Japan
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DOI：
10.1016/j.virol.2009.06.025
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发表时间：
2009-08-15
期刊：
影响因子：
3.7
通讯作者：
Kobayashi, Mutsuo
中科院分区：
文献类型：
--
作者：
Hoshino, Keita;Isawa, Haruhiko;Kobayashi, Mutsuo
我们从日本白纹伊蚊及其近缘种中分离到一种新的黄病毒。这种病毒被命名为伊蚊黄色病毒(AEFV)，只在蚊子细胞系中复制，并产生轻微的细胞病变效应。AEFV基因组为正向单链RNA，全长11,064个核苷酸，含有一个开放阅读框，编码3341个氨基酸，5‘和3’非翻译区分别为96和945个核苷酸。分别进行了分析。遗传和系统发育分析将AEFV与昆虫黄病毒分类，但不同于细胞融合剂(CFA)、卡米提河病毒和库蚊黄病毒。有趣的是，AEFV的部分序列与整合在白纹伊蚊基因组中的昆虫黄病毒相关核苷酸序列--细胞沉默因子(CsA)的序列具有显著的相似性。这些结果表明，AEFV是昆虫黄病毒的一个新成员，与伊蚊关系密切，可能与环孢菌素A有共同的起源。(C)2009 Elsevier Inc.保留所有权利。
We isolated a new flavivirus from Aedes albopictus mosquito and a related species in Japan. The Virus, designated Aedes flavivirus (AEFV), only replicated in a mosquito cell line and produced a mild cytopathic effect. The AEFV genome was positive-sense, single-stranded RNA, 11,064 nucleotides in length and contained a single open reading frame encoding a polyprotein of 3341 amino acids with 5' and 3' untranslated regions (UTRs) of 96 and 945 nucleotides. respectively. Genetic and phylogenetic analyses classified AEFV with the insect flavivirus, but distinct from Cell fusing agent (CFA), Kamiti river Virus and Culex flavivirus. interestingly, a partial sequence of AEFV showed significant similarity to that of Cell silent agent (CSA), the insect flavivirus-related nucleotide sequence integrated in the genome of A. albopictus. These results Suggest that AEFV is a new member of the insect flaviviruses, which are intimately associated with Aedes mosquitoes and may share a common origin with CSA. (C) 2009 Elsevier Inc. All rights reserved.