Periodontal tissue activation by vibration: Intermittent stimulation by resonance vibration accelerates experimental tooth movement in rats
Periodontal tissue activation by vibration: Intermittent stimulation by resonance vibration accelerates experimental tooth movement in rats
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DOI：
10.1016/j.ajodo.2006.01.046
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发表时间：
2008-04-01
影响因子：
3
通讯作者：
Mitani, Hideo
中科院分区：
文献类型：
--
作者：
Nishimura, Makoto;Chiba, Mirei;Mitani, Hideo
前言：加快正畸牙齿移动的速度应有助于缩短治疗时间。这将是有益的，因为长的治疗时间是正畸治疗的负面影响。在这项研究中，我们评估了机械刺激共振对牙齿移动的影响，我们显示牙周膜反应的细胞和分子机制。研究方法：将6周龄雄性Wistar大鼠的上颌第一磨牙通过使用膨胀弹簧移动到颊侧21天（n = 6，对照组），并测量牙齿移动量。在第0、7和14天，在正畸牙齿移动过程中，通过使用加载振动系统对第一磨牙施加额外的振动刺激（60 Hz，1.0 m/s（2）），持续8 min（n = 6，实验组）。在麻醉下处死动物，解剖每个上颌骨。标本经固定、脱钙、石蜡包埋。切片用于NF κ B B配体受体激活因子（RANKL）表达的免疫组织化学分析。用TRAP染色计数牙槽骨中破骨细胞的数量，用苏木精和伊红染色切片测量牙根吸收的量。结果：上颌第一磨牙的平均共振频率为61.02 ± 8.38Hz。实验组牙齿移动量明显大于对照组（P
Introduction: Accelerating the speed of orthodontic tooth movement should contribute to the shortening of the treatment period. This would be beneficial because long treatment times are a negative aspect of orthodontic treatment. In this study, we evaluated the effects of mechanical stimulation by resonance vibration on tooth movement, and we showed the cellular and molecular mechanisms of periodontal ligament responses. Methods: The maxillary first molars of 6-week-old male Wistar rats were moved to the buccal side by using an expansive spring for 21 days (n = 6, control group), and the amount of tooth movement was measured. Additional vibrational stimulation (60 Hz, 1.0 m/s(2)) was applied to the first molars by using a loading vibration system for 8 minutes on days 0, 7, and 14 during orthodontic tooth movement (n = 6, experimental group). The animals were killed under anesthesia, and each maxilla was dissected. The specimens were fixed, decalcified, and embedded in paraffin. Sections were used for immunohistochemical analysis of receptor activator of NF kappa B ligand (RANKL) expression. The number of osteoclasts in the alveolar bone was counted by using TRAP staining, and the amount of root resorption was measured in sections stained with hematoxylin and eosin. Results: The average resonance frequency of the maxillary first molar was 61.02 +/- 8.38 Hz. Tooth movement in the experimental group was significantly greater than in the control group (P