Pulse immunoassay for Candida albicans.
Pulse immunoassay for Candida albicans.
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白色念珠菌的脉冲免疫测定。
DOI：
10.1021/ac00286a049
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发表时间：
1985
影响因子：
7.4
通讯作者：
I. Karube
中科院分区：
文献类型：
--
作者：
H. Matsuoka;E. Tamiya;I. Karube
1998年Anal. 1985，57，1998-2002中所述的方法，弱酸如碳酸（CO2 + H2O）不会产生显著的pH变化。这在用缬氨霉素制备的ISFET的情况下观察到。不幸的是，在这一点上，尚不清楚为什么用不含缬氨霉素的PVC膜和不含缬氨霉素的KTPB或硅橡胶膜制备的装置通常显示出对CO2的更大响应，尽管可以推测缬氨霉素的存在以某种方式影响膜物质分配到门区，因此，通过使用ISFET的附加实验证实了上述模型，所述ISFET首先通过在碳酸氢钠水溶液中浸涂栅极区域来处理。一旦干燥，这些装置在门上具有固体碳酸氢钠薄膜，然后用PVC或硅橡胶膜重新涂覆。在水溶液中初始调节后，所有这些ISFET都显示出对CO2的大且快速的响应。我们得出结论，碳酸氢盐层在调节步骤期间再水合，并在门和聚合物膜之间形成更明确的层（具有更高的pH值）。
1998 Anal. Chem. 1985, 57, 1998-2002 a weak acid such as carbonic acid (C02+ H20) would not yield dramatic pH changes. This was observed in the case of the ISFETs prepared with valinomycin. Unfortunately, at this point it is not clear why devices prepared with PVC mem-branes without valinomycin and KTPB or silicone rubber membranes without valinomycin typically displayed larger responses to C02, although one can speculate that the presence of valinomycininfluences, in some way, the partitioning of membrane species into the gate region, and, therefore, the initial pHof this region.The above model was substantiated by additional experiments using ISFETs which were first treated by dip coating the gate region in an aqueous sodium bicarbonate solution. Once dried, these devices possessed a thin film of solid sodium bicarbonate on the gate and were then recoated with the PVC or silicone rubber membranes. Following initial conditioning in aqueous solutions, all of these ISFETs displayed large and rapid responses to C02. We conclude that the bicarbonate layer rehydrates during the conditioning step and forms a more well-defined layer (with higher pH) between the gate and polymeric membranes.