Serum Wisteria Floribunda Agglutinin-Positive Sialylated Mucin 1 as a Marker of Progenitor/Biliary Features in Hepatocellular Carcinoma.
Serum Wisteria Floribunda Agglutinin-Positive Sialylated Mucin 1 as a Marker of Progenitor/Biliary Features in Hepatocellular Carcinoma.
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DOI：
10.1038/s41598-017-00357-8
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发表时间：
2017-03-21
影响因子：
4.6
通讯作者：
Izumi N
中科院分区：
文献类型：
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作者：
Tamaki N;Kuno A;Matsuda A;Tsujikawa H;Yamazaki K;Yasui Y;Tsuchiya K;Nakanishi H;Itakura J;Korenaga M;Mizokami M;Kurosaki M;Sakamoto M;Narimatsu H;Izumi N
肝细胞癌的组织分子分类对预测预后具有重要的临床意义。然而，目前还没有建立一个可靠的血清标志物。本研究旨在探讨血清紫藤凝集素阳性唾液酸化粘蛋白1(WFA-sialylated MUC1)作为一种新的胆道标志物对有肝祖细胞(HPC)/胆道特征的肝细胞癌的诊断价值。共有144名接受完全射频消融治疗的原发性肝癌患者入选。血清WFA分析的MUC1900 μL/ml被确定为预测HPC/胆管特征免疫组化染色的最佳界值[唾液酸化的MUC1C19和细胞角蛋白19]。≥900型患者唾液酸化MUC1CK19阳性率明显高于WFA型MUC1C900型患者。此外，在WFA分析的MUC1900患者中，肝细胞癌复发的累积发生率显著更高，在多因素分析中，血清WFA分析的≥水平是肝细胞癌复发的独立预测因子。提示血清WFA分析的MUC1与肝细胞癌的组织学特征及治疗后复发有关，可作为判断肝细胞癌的HPC/胆道特征及预后的新指标。
Histological molecular classification of hepatocellular carcinoma (HCC) is clinically important for predicting the prognosis. However, a reliable serum marker has not been established. The aim of this study was to evaluate the diagnostic value of serum Wisteria Floribunda agglutinin-positive sialylated mucin 1 (WFA-sialylated MUC1), which is a novel biliary marker, as a marker of HCC with hepatic progenitor cell (HPC)/biliary features and of prognosis. A total of 144 consecutive patients who underwent complete radiofrequency ablation of primary HCC were enrolled. A serum WFA-sialylated MUC1 level of 900 μL/mL was determined as the optimal cutoff value for prediction of immunohistochemical staining for HPC/biliary features [sialylated MUC1 and cytokeratin 19 (CK19)]. Positive staining rate of sialylated MUC1 and CK19 was significantly higher in patients with WFA-sialylated MUC1 ≥900 than those with WFA-sialylated MUC1 <900. Furthermore, cumulative incidence of HCC recurrence was significantly higher in patients with WFA-sialylated MUC1 ≥900 and on multivariate analysis, serum WFA-sialylated MUC1 levels was an independent predictor of HCC recurrence. These results revealed that serum WFA-sialylated MUC1 was associated with histological feature of HCC and recurrence after curative therapy and it could be a novel marker of HPC/biliary features in HCC and of prognosis.