The insulin-like growth factor II/mannose 6-phosphate receptor is required for proliferin-induced angiogenesis.
The insulin-like growth factor II/mannose 6-phosphate receptor is required for proliferin-induced angiogenesis.
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DOI：
10.1210/endo.137.9.8756559
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发表时间：
1996-09
期刊：
影响因子：
4.8
通讯作者：
O. Volpert;D. Jackson;N. Bouck;D. Linzer
中科院分区：
文献类型：
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作者：
O. Volpert;D. Jackson;N. Bouck;D. Linzer
增殖素能刺激培养中的内皮细胞迁移和体内新生血管。以前的研究表明，增殖蛋白可以与胰岛素样生长因子II/甘露糖6-磷酸受体结合，这种结合可以被甘露糖6-磷酸阻断。我们现在发现，该受体在增殖素诱导的血管生成中起着重要作用。添加甘露糖6-磷酸可阻断增殖素与内皮细胞的结合，以及重组和胎盘来源的增殖素在体外刺激毛细血管内皮细胞迁移和诱导大鼠角膜新生血管的能力。与该受体在血管生成中的直接作用一致，胰岛素样生长因子II以及一种突变形式的胰岛素样生长因子II也刺激内皮细胞迁移和新生血管形成。
Proliferin stimulates endothelial cell migration in culture and neovascularization in vivo. Previous studies have demonstrated that proliferin can bind to the insulin-like growth factor II/mannose 6-phosphate receptor, and that binding can be blocked by mannose 6-phosphate. We have now found that this receptor plays an essential role in proliferin-induced angiogenesis. Proliferin binding to endothelial cells is blocked by the addition of mannose 6-phosphate, as is the ability of both recombinant and placental-derived proliferin to stimulate the migration of capillary endothelial cells in vitro and to induce neovascularization in the rat cornea. Consistent with a direct role of this receptor in angiogenesis, insulin-like growth factor II, as well as a mutant form of insulin-like growth factor II that binds to the insulin-like growth factor II/mannose 6-phosphate receptor but not to the insulin-like growth factor I receptor, also stimulate endothelial cell migration and neovascularization.