Detection of Autoantibodies to Tumour-Associated Antigens in Sera of Patients with Systemic Autoimmunity Using a Novel Protein Microblot Array
Detection of Autoantibodies to Tumour-Associated Antigens in Sera of Patients with Systemic Autoimmunity Using a Novel Protein Microblot Array
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DOI：
10.1111/j.1365-3083.2009.02257.x
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发表时间：
2009-06-01
影响因子：
3.7
通讯作者：
Bachmann, M. P.
中科院分区：
文献类型：
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作者：
Canzler, U.;Bartsch, H.;Bachmann, M. P.
众所周知，系统性自身免疫患者的血清含有针对核抗原的自身抗体。还已知具有全身性自身免疫的患者具有增加的肿瘤发展风险。有趣的是，肿瘤患者经常会产生自身抗体，并且潜在的肿瘤相关抗原越来越多。然而，目前尚不清楚全身性自身免疫患者是否也会产生针对肿瘤相关抗原的抗体。在这里，我们描述了一种新的多蛋白阵列的发展，使我们能够平行筛选30种不同的肿瘤相关抗原的自身抗体。使用这种新的检测方法，我们发现，与年龄匹配的对照组相比，全身性自身免疫患者中针对选定肿瘤相关抗原的自身抗体的频率增加了2至14倍。
It is well known that sera of patients with systemic autoimmunity contain autoantibodies to nuclear antigens. It is also known that patients with systemic autoimmunity have an increased risk for the development of tumours. Interestingly, tumour patients frequently develop autoantibodies and there is a growing list of potential tumour-associated antigens. It is, however, not known whether or not patients with systemic autoimmunity also develop antibodies to tumour-associated antigens. Here we describe the development of a novel multiprotein array allowing us to screen for autoantibodies to 30 different tumour-associated antigens in parallel. Using this novel assay, we found that the frequency of autoantibodies to the selected tumour-associated antigens is increased between 2- and 14-fold in patients with systemic autoimmunity compared with an age-matched control group.