Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase
Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase
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DOI：
10.1089/ars.2006.8.628
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发表时间：
2006-03-01
影响因子：
6.6
通讯作者：
Richardson, Arlan
中科院分区：
文献类型：
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作者：
Mele, James;Van Remmen, Holly;Richardson, Arlan
同时过表达铜/锌超氧化物歧化酶和过氧化氢酶[TG(SOD1/CAT)(+/o)]的转基因小鼠被用来评价这两个基因的过表达对氧化应激的影响。对这些转基因小鼠的鉴定表明，与野生型小鼠相比，转基因小鼠组织中的过氧化氢酶或铜/锌超氧化物歧化酶的活性高出两到四倍，而其他主要抗氧化酶的活性在转基因小鼠的组织中没有改变。与野生型成纤维细胞相比，来自TG(SOD1/CAT)(+/o)的小鼠胚胎成纤维细胞(MEF)和高表达铜/锌超氧化物歧化酶的MEF对百草枯细胞毒性的抗性更强。来自TG(SOD1/CAT)(+/o)的MEF对百草枯细胞毒性的抗性(最高可达2.25倍)高于单独表达铜/锌超氧化物歧化酶或过氧化氢酶的MEF。来自TG(CAT)(+/o)和TG(SOD1/CAT)(+/o)的MEF对过氧化氢细胞毒性的抗性相同。然而，无论是百草枯还是伽马射线，在整个动物存活率方面都没有显著差异。
Transgenic mice overexpressing both Cu/ZnSOD and catalase [Tg(SOD1/CAT)(+/o)] were used to evaluate the effects of overexpression of both genes against oxidative stress. Characterization of these transgenic mice revealed that catalase or Cu/ZnSOD activities were two- to fourfold higher in the tissues of transgenic mice compared to wild-type mice, and the activities of the other major antioxidant enzymes were not altered in the tissues of the transgenic mice. The murine embryonic fibroblasts (MEFs) from the Tg(SOD1/CAT)(+/o) and MEFs overexpressing Cu/ZnSOD were more resistant to paraquat cytotoxicity, relative to wild-type MEFs. The MEFs from Tg(SOD1/CAT)(+/o) tended to be more resistant (up to 2.25-fold) to paraquat cytotoxicity than MEFs overexpressing either Cu/ZnSOD or catalase alone. MEFs from Tg(CAT)(+/o) and Tg(SOD1/CAT)(+/o) were equally as resistant to hydrogen peroxide cytotoxicity. However, there were no significant differences in whole animal survival against either paraquat or gamma-radiation.