An ultrasensitive and dual-recognition SERS biosensor based on Fe3O4@Au-Teicoplanin and aptamer functionalized Au@Ag nanoparticles for detection of Staphylococcus aureus
An ultrasensitive and dual-recognition SERS biosensor based on Fe3O4@Au-Teicoplanin and aptamer functionalized Au@Ag nanoparticles for detection of Staphylococcus aureus
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基于 Fe3O4@Au-Teicoplanin 和适体功能化 Au@Ag 纳米颗粒的超灵敏双识别 SERS 生物传感器，用于检测金黄色葡萄球菌
DOI：
10.1016/j.talanta.2022.123648
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发表时间：
2022-07-20
期刊：
影响因子：
6.1
通讯作者：
Zhou, Nandi
中科院分区：
文献类型：
--
作者：
Qi, Xiaoyu;Ye, Yufei;Zhou, Nandi
以替考拉宁(TCP)功能化的金黄色葡萄球菌磁性纳米颗粒(Fe3O4@Au-TCPNPs)为捕获探针，金黄色葡萄球菌适体(APT)功能化的银纳米颗粒(Au@Ag-DTNb-APT NPs)为信号探针，构建了一种超灵敏、双重识别的金黄色葡萄球菌表面增强拉曼散射(SERS)生物传感器。采用绿色合成方法制备了Au纳米颗粒和Au@Ag纳米颗粒。特别是首次报道了壳聚糖(CS)还原Au@Ag纳米粒子的合成方法。基于Au纳米颗粒和Au@Ag纳米颗粒之间的热点效应，以及基于TCP和APT的双重识别能力，SERS生物传感器具有极高的灵敏度和特异度。在50min内，检测下限为1.09 cfu mL(-1)，线性范围为7.6×10(1)-7.6×10(7)cfu mL(-1)，无其他细菌干扰。此外，SERS生物传感器可用于牛奶和橙汁样品中金黄色葡萄球菌的检测。本研究提供了一种绿色、快速、超灵敏的检测金黄色葡萄球菌的方法，并探索了CS和TcP的高利用价值，在病原菌检测中具有广阔的应用前景。
An ultrasensitive and dual-recognition surface-enhanced Raman scattering (SERS) biosensor for Staphylococcus aureus (S. aureus) was constructed, which was based on teicoplanin (Tcp) functionalized gold-coated magnet nanoparticles (Fe3O4@Au-Tcp NPs) as capture probe and S. aureus aptamer (Apt) functionalized silver coated gold nanoparticles (Au@Ag-DTNB-Apt NPs) as signal probe. Both Au NPs and Au@Ag NPs were prepared by a green synthesis method. Especially, the synthesis method of Au@Ag NPs reduced by chitosan (CS) was first reported in this work. Due to the great SERS enhancement based on the hot spot effect between Au NPs and Au@Ag NPs, and the dual-recognition ability based on Tcp and Apt, the SERS biosensor was ultrasensitive and specific. A detection limit of 1.09 CFU mL(-1) with a broad dynamic linear (7.6 x 10(1)-7.6 x 10(7) CFU mL(-1)) was achieved within 50 min without interference by other bacteria. Moreover, the SERS biosensor could be applied for detection of S. aureus in milk and orange juice samples. This study provides a green, rapid and ultrasensitive method to detect S. aureus , and also explores the high utilization value of CS and Tcp, which has a broad application prospect in detection of pathogenic bacteria.