Long noncoding RNA and messenger RNA abnormalities in pediatric sepsis: a preliminary study
Long noncoding RNA and messenger RNA abnormalities in pediatric sepsis: a preliminary study
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DOI：
10.1186/s12920-020-0698-x
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发表时间：
2020-03-10
影响因子：
2.7
通讯作者：
Fang, Fang
中科院分区：
文献类型：
--
作者：
Bai, Zhenjiang;Li, Yiping;Fang, Fang
脓毒症是一种复杂的疾病，炎症反应失调，死亡率高。据报道，长链非编码rna （lncRNAs）在多种生物过程中发挥调控作用。然而，评估lncRNAs在儿童脓毒症中的功能的研究很少，目前对lncRNAs在儿童脓毒症中的作用的认识仍然有限。本研究基于全面的微阵列分析，探讨了儿童败血症患者和健康对照组之间lncRNAs和mrna的表达模式。方法采用LncRNA和mRNA芯片检测脓毒症组和对照组的LncRNA和mRNA表达情况。通过富集分析、受试者工作特征（ROC）曲线分析、共表达网络分析和实时定量PCR （qPCR）进一步解释鉴定出的异常表达mrna和lncRNAs。结果共鉴定出1488个差异表达lncrna和1460个差异表达mrna。构建了鉴定的lncrna和mrna的共表达网络。在该网络中，lncRNA lnc-RP11-1220 K2.2.1-7与mRNA CXCR1和CLEC4D相关；lncRNA lnc-ANXA3-2与mRNA CLEC4D相关；lncRNA lnc-TRAPPC5-1与mRNA DYSF和HLX相关；lncRNA lnc-ZNF638-1与mRNA DYSF和HLX相关。通过qPCR验证了4个lncrna和4个共表达mrna在儿童脓毒症患者和对照组之间的表达存在显著差异，验证了芯片结果。结论我们的研究有助于全面了解lncrna和mrna在儿童脓毒症中的作用，为后续的实验研究提供指导。此外，我们的研究也可能为儿科败血症的诊断和治疗提供潜在的候选lncrna和mrna。
Background Sepsis represents a complex disease with dysregulated inflammatory response and high mortality rate. Long noncoding RNAs (lncRNAs) have been reported to play regulatory roles in a variety of biological processes. However, studies evaluating the function of lncRNAs in pediatric sepsis are scarce, and current knowledge of the role of lncRNAs in pediatric sepsis is still limited. The present study explored the expression patterns of both lncRNAs and mRNAs between pediatric sepsis patients and healthy controls based on a comprehensive microarray analysis. Methods LncRNA and mRNA microarray was used to detect the expression of lncRNAs and mRNAs in the septic and control groups. Aberrantly expressed mRNAs and lncRNAs identified were further interpreted by enrichment analysis, receiver operating characteristic (ROC) curve analysis, co-expression network analysis, and quantitative real-time PCR (qPCR). Results A total of 1488 differetially expressed lncRNAs and 1460 differentially expressed mRNAs were identified. A co-expression network of the identified lncRNAs and mRNAs was constructed. In this network, lncRNA lnc-RP11-1220 K2.2.1-7 is correlated with mRNA CXCR1 and CLEC4D; lncRNA lnc-ANXA3-2 is correlated with mRNA CLEC4D; lncRNA lnc-TRAPPC5-1 is correlated with mRNA DYSF and HLX; lncRNA lnc-ZNF638-1 is correlated with mRNA DYSF and HLX. Significantly different expressions between pediatric sepsis patients and controls were validated by qPCR for the 4 lncRNAs and 4 co-expressed mRNAs, validating the microarray results. Conclusions Our study contributes to a comprehensive understading of the involvment of lncRNAs and mRNAs in pediatric sepsis, which may guide subsequent experimental research. Furthermore, our study may also provide potential candidate lncRNAs and mRNAs for the diagnosis and treatment of pediatric sepsis.