N-methyl-N-nitrosourea-induced rat mammary tumors. Hormone responsiveness but lack of spontaneous metastasis.
N-methyl-N-nitrosourea-induced rat mammary tumors. Hormone responsiveness but lack of spontaneous metastasis.
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N-甲基-N-亚硝基脲诱导的大鼠乳腺肿瘤。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
A. Neville
中科院分区：
文献类型：
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作者：
J. Williams;B. Gusterson;J. Humphreys;P. Monaghan;R. Coombes;P. Rudland;A. Neville
N-甲基-N-亚硝基脲（MNU）在50 ~ 55日龄大鼠中诱导70%的F344/N和91%的W/ICRF近交系雌性乳腺肿瘤，平均潜伏期分别为149天和93天。减少MNU剂量对W/ICRF大鼠的肿瘤发生率没有影响。在乳腺肿瘤中，98%在组织学上被分类为腺癌，其生长呈进行性。非乳腺原发肿瘤的发生率较低。经组织学检查，未发现乳腺或其他原发肿瘤转移的证据。没有发现肿瘤诱导的高钙血症的证据。在给予MNU时进行卵巢切除可阻止肿瘤的发展；当至少1个肿瘤/动物可触及时，切除卵巢会导致生长延迟或退化。所有mnu诱导的乳腺肿瘤和7,12-二甲基苯[a]蒽诱导的乳腺肿瘤都含有相似浓度的细胞质雌激素受体（ER），并且在组织学上没有区别。mnu诱导的F344大鼠肿瘤可移植，并通过三次移植保留内质网。
N-Methyl-N-nitrosourea (MNU) given iv to rats 50-55 days old induced mammary tumors in 70% of F344/N and 91% W/ICRF inbred females with mean latency periods of 149 and 93 days, respectively. Reduction of the MNU dose did not affect tumor incidence in W/ICRF rats. Of the mammary tumors, 98% were classified histologically as adenocarcinomas, which grew progressively. Primary tumors of nonmammary origin were detected at low incidence. Upon histologic examination, no evidence was found for metastases of either the mammary or other primary tumors. No evidence for tumor-induced hypercalcemia was found. Oophorectomy at the time of MNU administration prevented tumor development; oophorectomy when at least 1 tumor/animal was palpable caused growth delay or regression. All MNU-induced and 7,12-dimethylbenz[a]anthracene-induced mammary tumors tested contained cytoplasmic estrogen receptor (ER) at similar concentrations and were indistinguishable histologically. MNU-induced tumors in F344 rats were transplantable and retained ER through three transplantations.