SINGLE-CHANNEL DOSE-RESPONSE STUDIES IN SINGLE, CELL-ATTACHED PATCHES
SINGLE-CHANNEL DOSE-RESPONSE STUDIES IN SINGLE, CELL-ATTACHED PATCHES
复制标题
DOI：
10.1016/s0006-3495(91)82095-1
复制
发表时间：
1991-09-01
影响因子：
3.4
通讯作者：
AUERBACH, A
中科院分区：
文献类型：
--
作者：
AUERBACH, A
设计了一种用于细胞贴附贴片离子通道电流的剂量响应研究的方法。在贴片移液器的尖端填充含有一种浓度的配体的溶液，然后回填另一种。膜上的配体浓度通过圆锥体中扩散的方程描述为时间的函数，允许将响应与时间数据转换为剂量-响应曲线。对于非洲爪蟾肌细胞胆碱能受体，使用这种方法的例子给出了几个浓度依赖性反应，包括封锁由局部麻醉剂QX-222，激活乙酰胆碱，和钠离子的电流振幅的调制。分析非平稳信道动力学的几种方法，包括伪平稳的方法，使用区间似然最大化。
A method for carrying out dose-response studies of ion channel currents in cell-attached patches has been devised. Patch pipettes are filled at the tip with a solution containing one concentration of ligand and then backfilled with another. The concentration of ligand at the membrane is described as a function of time by the equation for diffusion in a cone, allowing response vs. time data to be transformed into a dose-response curve. For Xenopus myocyte cholinergic receptors, examples of the use of this method are given for several concentration-dependent reactions including blockade by the local anesthetic QX-222, activation by acetylcholine, and modulation of current amplitude by sodium ions. Several methods of analyzing the nonstationary channel kinetics are presented, including a pseudo-stationary approach that uses interval likelihood maximization.