Increased N-acetyltaurine In serum and urine after endurance exercise in human
Increased N-acetyltaurine In serum and urine after endurance exercise in human
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人体耐力运动后血清和尿液中 N-乙酰牛磺酸增加
DOI：
10.1007/978-3-319-15126-7_5
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发表时间：
2015
影响因子：
--
通讯作者：
Yasushi Matsuzaki
中科院分区：
文献类型：
--
作者：
Teruo Miyazaki;Keisuke Ishikura;Akira Honda;Song-Gyu Ra;Shoichi Komine;Yasunori Miyamoto;Hajime Ohmori;Yasushi Matsuzaki
n -乙酰牛磺酸（NAT）是牛磺酸与乙酸结合的一种新型代谢物，据报道在酒精分解代谢过程中尿中含量增加。因为耐力运动也会增加醋酸酯的生成，所以在运动过程中可能会产生NAT。本研究旨在测定耐力运动后血清和尿液中的NAT。采用高效液相色谱-质谱联用系统测定短暂性运动或全程马拉松比赛后血清和尿液中牛磺酸和NAT水平。此外，在培养的骨骼肌细胞（C2C12肌管）暴露于牛磺酸和醋酸盐中，对NAT的合成进行了评价。与马拉松前相比，全程马拉松后血清牛磺酸和NAT浓度立即显著升高，一天后恢复到正常水平。短暂运动后24小时内尿中牛磺酸和NAT的排泄量明显高于运动前。在培养细胞中，同时暴露于牛磺酸和醋酸盐后，NAT向培养基的排泄量显著增加。本研究证实了耐力运动后血清和尿液中NAT的显著升高，提示运动过程中牛磺酸可能与骨骼肌中过量的乙酰基结合。
N-acetyltaurine (NAT) that is a novel metabolite of taurine conjugated with acetate has been reported to increase in urine during alcohol catabolism. Because acetate production would be also increased by endurance exercise, NAT might be produced during the exercise. This study aimed to determine the NAT in serum and urine after endurance exercises. Serum and urinary levels of taurine and NAT after a transient exercise or full-marathon race were measured by HPLC-MS/MS system. In addition, the synthesis of NAT was evaluated in cultured skeletal muscle cell (C2C12 myotube) exposed to taurine and acetate. Serum taurine and NAT concentrations were significantly increased immediately after full-marathon compared to those before the marathon, and recovered to the normal levels after a day. Urinary excretions of taurine and NAT were markedly higher in 24-h period after the transient exercise compared to those before the exercise. In culture cell, the excretion of NAT to medium was significantly increased following the exposing to both taurine and acetate. The present study could confirm the significant increase of NAT in serum and urine after endurance exercise, and it is suggesting that taurine may conjugate with excess acetyl-group in the skeletal muscles during exercise.