ISOLATION, CHARACTERIZATION, AND PHYSICAL MAPPING OF TEMPERATURE-SENSITIVE MUTANTS OF VACCINIA VIRUS
ISOLATION, CHARACTERIZATION, AND PHYSICAL MAPPING OF TEMPERATURE-SENSITIVE MUTANTS OF VACCINIA VIRUS
复制标题
DOI：
10.1016/0042-6822(83)90268-4
复制
发表时间：
1983-01-01
期刊：
影响因子：
3.7
通讯作者：
SPIZZ, G
中科院分区：
文献类型：
--
作者：
CONDIT, RC;MOTYCZKA, A;SPIZZ, G
新的温度敏感型痘苗病毒突变体（39）已被分离出来，扩大了以前报道的突变体的收集，共65。65个突变体被分配到32个互补组，主要是基于先前描述的定性斑点试验。在非允许温度下测定每个互补组的代表的DNA和蛋白质合成，揭示了1个新的DNA阴性互补组，3个新的组含有在后期蛋白质合成中有缺陷的突变体和10个新的组含有以正常方式合成DNA和蛋白质的突变体。使用来自野生型病毒的克隆DNA片段，用65个突变体中的29个实现了标记拯救。这29个突变体一起代表32个互补组中的20个。一个初步的物理地图的突变。
New temperature-sensitive mutants (39) of vaccinia virus have been isolated, expanding a previously reported collection of mutants to a total of 65. The 65 mutants were assigned to 32 complementation groups, based primarily on a qualitative spot test described previously. Representatives of each complementation group were assayed for DNA and protein synthesis at the nonpermissive temperature, revealing 1 new DNA-negative complementation group, 3 new groups which contain mutants defective in late protein synthesis and 10 new groups containing mutants which synthesize DNA and protein in a normal fashion. Marker rescue was achieved with 29 of the 65 mutants using cloned DNA fragments from wild-type virus. These 29 mutants together represent 20 of the 32 complementation groups. A preliminary physical map of the mutants is presented.