Neovascular expression of E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 in human atherosclerosis and their relation to intimal leukocyte content
Neovascular expression of E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 in human atherosclerosis and their relation to intimal leukocyte content
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DOI：
10.1161/01.cir.93.4.672
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发表时间：
1996-02-15
期刊：
影响因子：
37.8
通讯作者：
Alpers, CE
中科院分区：
文献类型：
--
作者：
OBrien, KD;McDonald, TO;Alpers, CE
背景白细胞募集是动脉粥样硬化形成的早期事件，近年来发现白细胞粘附分子E-选择素、细胞间粘附分子-1（ICAM-1）和血管细胞粘附分子-1（VCAM-1）在动脉粥样硬化形成中起重要作用。然而，没有以前的研究已经评估了这三种分子在不同的网站内的动脉内膜或斑块白细胞content.Methods和结果的分布进行了免疫组化99冠状动脉段（34个控制和65动脉粥样硬化斑块），以确定E-选择素，ICAM-1，VCAM-1，巨噬细胞，平滑肌细胞和T淋巴细胞。对于每个节段，在动脉管腔、内膜新生血管和内膜非内皮细胞上确定是否存在粘附分子。以0 - 3的半定量量表对每个节段的内膜巨噬细胞和T淋巴细胞密度进行评分。在动脉粥样硬化斑块中，E-选择素、ICAM-1和VCAM-1在斑块新生血管中的患病率是它们在动脉管腔内皮中的患病率的两倍。E-选择素是唯一的粘附分子，其在动脉管腔内皮细胞上的表达在斑块中比在对照节段中更普遍。斑块内膜巨噬细胞密度增加与新生血管VCAM-1表达相关（P
Background Leukocyte recruitment is an early event in atherogenesis, and the leukocyte adhesion molecules E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) recently have been detected in human atherosclerosis. However, no previous study has evaluated either the distribution of these three molecules at different sites within the arterial intima or their relation to plaque leukocyte content.Methods and Results Immunohistochemistry was performed on 99 coronary artery segments (34 controls and 65 with atherosclerotic plaque) to identify E-selectin, ICAM-1, VCAM-1, macrophages, smooth muscle cells, and T lymphocytes. For each segment, the presence or absence of adhesion molecule was determined at the arterial lumen, on intimal neovasculature, and on intimal nonendothelial cells. Each segment was scored for intimal macrophage and T-lymphocyte densities on a semiquantitative scale of 0 to 3. In atherosclerotic plaques, the prevalences of E-selectin, ICAM-1, and VCAM-1 on plaque neovasculature were twofold higher than their prevalences on arterial luminal endothelium. E-selectin was the only adhesion molecule for which expression on arterial luminal endothelial cells was more prevalent in plaques than in control segments. Increased plaque intimal macrophage density was associated with expression of VCAM-1 on neovasculature (P