Osmotic-Induced Reconfiguration and Activation in Membranized Coacervate-Based Protocells.
Osmotic-Induced Reconfiguration and Activation in Membranized Coacervate-Based Protocells.
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DOI：
10.1021/jacs.3c02540
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发表时间：
2023-04
影响因子：
15
通讯作者：
Yanwen Zhang;Zefeng Wang;Mei Li;Can Xu;N. Gao;Zhuping Yin;Kemin Wang;Stephen Mann;Jianbo Liu
中科院分区：
文献类型：
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作者：
Yanwen Zhang;Zefeng Wang;Mei Li;Can Xu;N. Gao;Zhuping Yin;Kemin Wang;Stephen Mann;Jianbo Liu
设计和构建具有刺激反应和稳态调节功能的合成原细胞是合成原生物学面临的一个重要挑战。在这里，我们朝着构建能够促进膜通透性增加和触发内源性酶反应的低渗应激诱导的体积反应的模型原细胞迈出了一步。我们描述了一种简便的自我转化过程，用于构建单室或多室的分子拥挤的原始细胞，其基础是脂质包裹的凝聚体液滴渗透重新配置为多隔室的凝聚体小泡。低渗肿胀扩大了细胞膜的通透性，增加了跨膜运输，从而可以通过渗透诱导的扩张在原细胞内触发和增强基于蛋白酶的水解和酶的级联反应。具体地说，我们演示了如何利用肿胀的凝聚小泡中一氧化氮(NO)的增加来诱导胸主动脉环血管的体外扩张。我们的方法为设计可重构的模型原始细胞提供了机会，这些细胞能够根据环境渗透压的变化进行自我平衡的体积调节、动态的结构重组和适应性功能，并可能在生物医学、细胞诊断和生物工程中得到应用。
The design and construction of synthetic protocells capable of stimuli response and homeostatic regulation is an important challenge for synthetic protobiology. Here, we develop a step toward the construction of model protocells capable of a hypotonic stress-induced volume response that facilitates an increase in membrane permeability and the triggering of endogenous enzyme reactions. We describe a facile self-transformation process for constructing single- or multichambered molecularly crowded protocells based on the osmotic reconfiguration of lipid-coated coacervate droplets into multicompartmentalized coacervate vesicles. Hypotonic swelling broadens membrane permeability and increases transmembrane transport such that protease-based hydrolysis and enzyme cascades can be triggered and enhanced within the protocells by osmotically induced expansion. Specifically, we demonstrate how the enhanced production of nitric oxide (NO) within the swollen coacervate vesicles can be used to induce in vitro blood vessel vasodilation in thoracic artery rings. Our approach provides opportunities for designing reconfigurable model protocells capable of homeostatic volume regulation, dynamic structural reorganization, and adaptive functionality in response to changes in environment osmolarity, and could find applications in biomedicine, cellular diagnostics, and bioengineering.